LOCUS       sequence-155711-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Kaberniuk AA, Shemetov AA, Verkhusha VV
  TITLE     A bacterial phytochrome-based optogenetic system controllable with 
            near-infrared light.
  JOURNAL   Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864.
  PUBMED    27159085
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 9. doi: 10.1038/nmeth.3864."
COMMENT     Sequence Label: pKA-207I10
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..752)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MTRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     660..679
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
      121 gcggccgctt aggacccact ttcacattta agttgttttt ctaatccgca tatgatcaat
      181 tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt
      241 cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga
      301 tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat
      361 aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt
      421 tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt
      481 agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct
      541 tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc
      601 aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg
      661 gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg
      721 ttaatcactt tacttttatc taatctagtc atggacccac cagcacttct agaactaccg
      781 ccggcactac cgccagcact cccgccggcg gacccaccag cactcttgta cagctcgtcc
      841 atgccgccgg tgg
//
