LOCUS       sequence-155712-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Mu-gam under the pBAD promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Cui L, Bikard D
  TITLE     Consequences of Cas9 cleavage in the chromosome of Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 8. pii: gkw223.
  PUBMED    27060147
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 8. pii: gkw223."
COMMENT     Sequence Label: pLC13
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(655..672)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt gggctagcga
       61 attcgagctc ggtaacttta agaaggagat ataccatggc taaaccagca aaacgtatca
      121 agagtgccgc agcggcttat gtgccacaaa accgcgatgc ggtgattacc gatattaaac
      181 gcatcgggga tttacagcgc gaagcatcac gtctggaaac ggaaatgaat gatgccatcg
      241 cggaaattac ggagaaattt gcggcccgga ttgcaccgat taaaaccgat attgaaaccc
      301 tttcaaaagg cgttcaggga tggtgtgaag cgaaccgcga cgaactgacg aacggcggca
      361 aagtgaagac ggcgaatctt gtcaccggtg atgtatcgtg gcgggtccgt ccaccatcag
      421 taagtattcg tggtatggat gcagtgatgg aaacgctgga gcgtcttggc cggcaacgct
      481 ttattcgcac gaagcaggaa atcaacaagg aagcgatttt actggaaccg aaagcggtcg
      541 caggcgttgc cggaattaca gttaaatcag gcattgagga tttttctatt attccatttg
      601 aacaggaagc cggtatttaa ttggctgttt tggcggatga gagaagattt tcagcctgat
      661 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
      721 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
      781 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatca
//
