LOCUS       sequence-155716-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GFP under the sulA promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Cui L, Bikard D
  TITLE     Consequences of Cas9 cleavage in the chromosome of Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 8. pii: gkw223.
  PUBMED    27060147
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 8. pii: gkw223."
COMMENT     Sequence Label: pZA31-sulA-GFP
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(294..322)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtattcttgt gatttataga tccgcttata ggagaggctt ttcataaaat tccttttaaa
       61 atcataacat aaaagaatga ttcacattaa cggatccgtt aactacgaaa ataggcaact
      121 tattcttaag gggcaagatt aatttatgtt ttcccgtcac caacgacaaa atttgcgagg
      181 ctctttccga aaatagggtt gatctttgtt gtcactggat gtactgtaca tccatacagt
      241 aactcacagg ggctggattg attatgagta aaggagaaga acttttcact ggagttgtcc
      301 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      361 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      421 tacctgttcc atggccaaca cttgtcacta ctttcgcgta tggtcttcaa tgctttgcga
      481 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      541 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      601 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      661 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      721 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
//
