LOCUS       sequence-155717-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tracing neuronal projection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Delwig A, Larsen DD, Yasumura D, Yang CF, Shah NM, Copenhagen DR
  TITLE     Retinofugal Projections from Melanopsin-Expressing Retinal Ganglion 
            Cells Revealed by Intraocular Injections of Cre-Dependent Virus.
  JOURNAL   PLoS One. 2016 Feb 19;11(2):e0149501. doi: 
            10.1371/journal.pone.0149501. eCollection 2016.
  PUBMED    26895233
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-19"; volume: "11(2):e0149501. doi"; pages: " 
            10.1371/journal.pone.0149501. eCollection 2016"
COMMENT     Sequence Label: AAV-flex-plap
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          105..483
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     protein_bind    complement(590..623)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(634..667)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc
       61 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata
      121 gagtctatag gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat
      181 ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct
      241 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt
      301 ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa
      361 tagcagctac aatccagcta ccattctgct tttattttat ggttgggata aggctggatt
      421 attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca
      481 cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga
      541 ttcgaacatc gatagcgacg cgtggacggt acccttcagg tcgacgtcga taacttcgta
      601 tagcatacat tatacgaagt tatccgaatc gcaataactt cgtataggat actttatacg
      661 aagttatttg gcgcgccaaa tttaaataac gcaacgtacc ttcgaggggg cccaaggaat
      721 tctcagggag cagtggccgt ctccagcagc agcagggtcc cggccagcag aggaagcaac
      781 gcggggacca cggaccgccc cgggtgcgcg gcgtcggtgg tgccggcggg gggcgccagg
      841 tcgcaggcgg tgtagggctc caggcaggcg gcgaaggcca tgacgtgcgc tatgaaggtc
      901 tgctcctgca cgccgtg
//
