LOCUS       sequence-155719-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  QF2 HACK backbone.  Contains QF2-hsp70, but no gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pHACK-QF2
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(155..188)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctgcgcttg tttatttgct tagctttcgc ttagcgacgt gttcactttg cttgtttgaa
       61 ttgaattgtc gctccgtaga cgaagcgcct ctatttatac tccggcgcta attgaattag
      121 ctctaattga attagtctct aattgaatga cgtcataact tcgtatagca tacattatac
      181 gaagttatag atcactagtg atctgaattc attttcaacg tcctcgatag tatagtggtt
      241 agtatccccg cctgtcacgc gggagaccgg ggttcaattc cccgtcgggg agaatctgtg
      301 attctttttt tttttctttt actttgttat ataaacaatt tttgttttaa ttgaatctaa
      361 tttgccattg cttttaggaa tctcaggcat ccagcaagcg tttgtccgcc gaatcgccca
      421 tcagtgaaga agatcctgtg gcggctacga aaatctcccc ggccatgtcg gcctccacct
      481 ccagcgaaaa acccatcagc gagctggcca cctctgtgct gacccaccgc tttccagact
      541 ccacctcctc acccggcgaa catggccttg gacgaatgca gttgtcgatc cgctacagcg
      601 cccagcgtca aaaactagac gtgaccatac acaaaatcca gaagatacca cttcgcgatc
      661 ccagcaatat ccccgatccg tatgttaagc tgtatctgtt gcctggacgc accaaggagt
      721 cgaaacgcaa gacgagcgtg atcaaggaca actgcaaccc cgtctacgat gcatcctttg
      781 agtacctgat ttccattgcc gaactcaggc agac
//
