LOCUS       sequence-155720-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  QF2 HACK backbone.  Contains QF2-hsp70, but no gRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pHACK-QF2
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(342..417)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(436..856)
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
ORIGIN
        1 atagtattct gagtgtcgta ttgagtctga gtgagacagc gatatgattg ttgattaacc
       61 cttagcatgt ccgtggggtt tgtaactagc ttacgtcaga attaattaag catacgcatt
      121 aagcgaacat taaaaagatg tgaaaacata actattatgt ctaaataaac acacgtcaga
      181 tgtatgtacg tcaacggaaa accattgtct atatattaca attactaaat acataccaaa
      241 ttgaatacat attgatgaaa aataataaat actggcgaaa gcaaaaaaac gaaacatttt
      301 tattttattg aacaactctc aggctccagg taggcaaaaa agcaccgact cggtgccact
      361 ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct ctaaaacagg
      421 tcttctcgaa gaccccgacg ttaaattgaa aataggtcta tatatacgaa ctgagtctgg
      481 aaaaagaagt tgagaattat aaaaagtagt gagcactggc gccctctctg cttggcgagc
      541 taaccttttc gcctcttggc tgagtaggtg gcgtttcatt ctactctgta aaattaatgt
      601 agaattgaaa cactatggtc aaaaaatact taggggcata agttataaaa cgtatgaaat
      661 gaatttttat caacctgggc tattcaaaaa ttttcgaatt attttatgta ttttttttaa
      721 tcgtttttca ttataggtta aaatacactt taaaaggaat tctttcctgt aaaataaata
      781 taaataaata tgctttattg acagaaaatt tgatgttttt gtatttgagt aggagcaatc
      841 acaggtgagc aaaaaagcac cgactcggtg ccact
//
