LOCUS       sequence-155722-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and 
            gRNAs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Lin CC, Potter CJ
  TITLE     Editing Transgenic DNA Components by Inducible Gene Replacement in 
            Drosophila melanogaster.
  JOURNAL   Genetics. 2016 Jun 22. pii: genetics.116.191783.
  PUBMED    27334272
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Jun 22. pii: genetics.116.191783."
COMMENT     Sequence Label: pHACK-GAL4>QF2
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(342..417)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atagtattct gagtgtcgta ttgagtctga gtgagacagc gatatgattg ttgattaacc
       61 cttagcatgt ccgtggggtt tgtaactagc ttacgtcaga attaattaag catacgcatt
      121 aagcgaacat taaaaagatg tgaaaacata actattatgt ctaaataaac acacgtcaga
      181 tgtatgtacg tcaacggaaa accattgtct atatattaca attactaaat acataccaaa
      241 ttgaatacat attgatgaaa aataataaat actggcgaaa gcaaaaaaac gaaacatttt
      301 tattttattg aacaactctc aggctccagg taggcaaaaa agcaccgact cggtgccact
      361 ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct ctaaaactcc
      421 agctttctca gaatacacga cgttaaattg aaaataggtc tatatatacg aactgagtct
      481 ggaaaaagaa gttgagaatt ataaaaagta gtgagcactg gcgccctctc tgcttggcga
      541 gctaaccttt tcgcctcttg gctgagtagg tggcgtttca ttctactctg taaaattaat
      601 gtagaattga aacactatgg tcaaaaaata cttaggggca taagttataa aacgtatgaa
      661 atgaattttt atcaacctgg gctattcaaa aattttcgaa ttattttatg tatttttttt
      721 aatcgttttt cattataggt taaaatacac tttaaaagga attctttcct gtaaaataaa
      781 tataaataaa tatgctttat tgacagaaaa tttgatgttt ttgtatttga gtaggagcaa
      841 tcacaggtga g
//
