LOCUS       sequence-155723-         695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
  TITLE     A multi-step model for facilitated unwinding of the yeast U4/U6 RNA 
            duplex.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 
            10.1093/nar/gkw686. Epub 2016 Aug 2.
  PUBMED    27484481
REFERENCE   2  (bases 1 to 695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw686"; journalName: "Nucleic Acids Res"; date: 
            "2016-12-15- 15"; volume: "44"; issue: "22"; pages: "10912-10928"
COMMENT     Sequence Label: pRS314-U6-G96C/A97C/U100C/U101C
FEATURES             Location/Qualifiers
     source          1..695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..36
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    153..174
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(189..194,195..212,213..219)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    227..243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     232..254
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     251..267
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     251..267
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        288..306
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     334..350
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     335..351
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(669..685)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
       61 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      121 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
      181 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      241 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
      301 taaagggaac aaaagctggt accgggcccc ccctcgaggt cgacggtatc gataagcttg
      361 atatcgaatt cttcggctac tataaataaa tgtttttttc gcaactatgt gttcgcgaag
      421 taacccttcg tggacatttg gtcaatttga aacaatacag agatgatcag cagttcccct
      481 gcataaggat gaaccgtttt acaaaccgac ctatttcgtt tttttttatc atcgagtgaa
      541 gtatcgtgac ttgtacattt gaagataccc agcgtacagc agtgtatctt tatcttcctg
      601 tatgatatag ataactaaca tctcgaatag aaaattgtct cgcgttcgaa cctaagcctt
      661 tgctatcgga tccactagtt ctagagcggc cgcca
//
