LOCUS       sequence-155724-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
  TITLE     A multi-step model for facilitated unwinding of the yeast U4/U6 RNA 
            duplex.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 
            10.1093/nar/gkw686. Epub 2016 Aug 2.
  PUBMED    27484481
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw686"; journalName: "Nucleic Acids Res"; date: 
            "2016-12-15- 15"; volume: "44"; issue: "22"; pages: "10912-10928"
COMMENT     Sequence Label: pRS314-U6-A34G/A35G/G96C/A97C
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..60
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    177..198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(213..218,219..236,237..243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    251..267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     256..278
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     275..291
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     275..291
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        312..330
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     358..374
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     359..375
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(693..709)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(693..709)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
       61 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
      121 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
      181 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
      241 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
      301 gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggtaccggg ccccccctcg
      361 aggtcgacgg tatcgataag cttgatatcg aattcttcgg ctactataaa taaatgtttt
      421 tttcgcaact atgtgttcgc gaagtaaccc ttcgtggaca tttggtcggt ttgaaacaat
      481 acagagatga tcagcagttc ccctgcataa ggatgaaccg ttttacaaac cgatttattt
      541 cgtttttttt tatcatcgag tgaagtatcg tgacttgtac atttgaagat acccagcgta
      601 cagcagtgta tctttatctt cctgtatgat atagataact aacatctcga atagaaaatt
      661 gtctcgcgtt cgaacctaag cctttgctat cggatccact agttctagag cggc
//
