LOCUS       sequence-155725-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo 
            studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
  TITLE     A multi-step model for facilitated unwinding of the yeast U4/U6 RNA 
            duplex.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 
            10.1093/nar/gkw686. Epub 2016 Aug 2.
  PUBMED    27484481
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw686"; journalName: "Nucleic Acids Res"; date: 
            "2016-12-15- 15"; volume: "44"; issue: "22"; pages: "10912-10928"
COMMENT     Sequence Label: pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..37
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    154..175
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(190..195,196..213,214..220)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    228..244
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     233..255
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     252..268
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     252..268
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        289..307
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     335..351
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     336..352
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(670..686)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(670..686)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
       61 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      121 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      181 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      241 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctcgaaa ttaaccctca
      301 ctaaagggaa caaaagctgg taccgggccc cccctcgagg tcgacggtat cgataagctt
      361 gatatcgaat tcttcggcta ctataaataa atgttttttt cgcaactatg tgttcgcgaa
      421 gtaacccttc gtggacattt ggtcggtttg aaacaataca gagatgatca gcagttcccc
      481 tgcataagga tgaaccgttt tacaaaccga cctatttcgt ttttttttat catcgagtga
      541 agtatcgtga cttgtacatt tgaagatacc cagcgtacag cagtgtatct ttatcttcct
      601 gtatgatata gataactaac atctcgaata gaaaattgtc tcgcgttcga acctaagcct
      661 ttgctatcgg atccactagt tctagagcgg ccgc
//
