LOCUS       sequence-155726-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 telestem mutant (A34G/A35G) for in vivo studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
  TITLE     A multi-step model for facilitated unwinding of the yeast U4/U6 RNA 
            duplex.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 
            10.1093/nar/gkw686. Epub 2016 Aug 2.
  PUBMED    27484481
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw686"; journalName: "Nucleic Acids Res"; date: 
            "2016-12-15- 15"; volume: "44"; issue: "22"; pages: "10912-10928"
COMMENT     Sequence Label: pRS314_U6_A34G_A35G
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..52
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    169..190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(205..210,211..228,229..235)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    243..259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     248..270
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     267..283
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     267..283
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        304..322
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     350..366
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     351..367
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(685..701)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(685..701)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
       61 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      121 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      181 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      241 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
      301 cgaaattaac cctcactaaa gggaacaaaa gctggtaccg ggccccccct cgaggtcgac
      361 ggtatcgata agcttgatat cgaattcttc ggctactata aataaatgtt tttttcgcaa
      421 ctatgtgttc gcgaagtaac ccttcgtgga catttggtcg gtttgaaaca atacagagat
      481 gatcagcagt tcccctgcat aaggatgaac cgttttacaa agagatttat ttcgtttttt
      541 tttatcatcg agtgaagtat cgtgacttgt acatttgaag atacccagcg tacagcagtg
      601 tatctttatc ttcctgtatg atatagataa ctaacatctc gaatagaaaa ttgtctcgcg
      661 ttcgaaccta agcctttgct atcggatcca ctagttctag agcggc
//
