LOCUS       sequence-155727-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 telestem mutant (G96C/A97C) for in vivo studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
  TITLE     A multi-step model for facilitated unwinding of the yeast U4/U6 RNA 
            duplex.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 
            10.1093/nar/gkw686. Epub 2016 Aug 2.
  PUBMED    27484481
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw686"; journalName: "Nucleic Acids Res"; date: 
            "2016-12-15- 15"; volume: "44"; issue: "22"; pages: "10912-10928"
COMMENT     Sequence Label: pRS314_U6_G96C_A97C
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     129..146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    263..284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(299..304,305..322,323..329)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    337..353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     342..364
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     361..377
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     361..377
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        398..416
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     444..460
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     445..461
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(779..795)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(779..795)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ggttcctgnc cttttgctgg ccttttgctc acatgttctt tcntgcgtta tcccctgatt
       61 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      121 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      181 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      241 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      301 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      361 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
      421 aaaagctggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
      481 cttcggctac tataaataaa tgtttttttc gcaactatgt gttcgcgaag taacccttcg
      541 tggacatttg gtcaatttga aacaatacag agatgatcag cagttcccct gcataaggat
      601 gaaccgtttt acaaaccgat ttatttcgtt tttttttatc atcgagtgaa gtatcgtgac
      661 ttgtacattt gaagataccc agcgtacagc agtgtatctt tatcttcctg tatgatatag
      721 ataactaaca tctcgaatag aaaattgtct cgcgttcgaa cctaagcctt tgctatcgga
      781 tccactagtt ctagagcggc
//
