LOCUS       sequence-155728-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 telestem mutant (U100C/U101C) for in vivo studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Rodgers ML, Didychuk AL, Butcher SE, Brow DA, Hoskins AA
  TITLE     A multi-step model for facilitated unwinding of the yeast U4/U6 RNA 
            duplex.
  JOURNAL   Nucleic Acids Res. 2016 Dec 15;44(22):10912-10928. doi: 
            10.1093/nar/gkw686. Epub 2016 Aug 2.
  PUBMED    27484481
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkw686"; journalName: "Nucleic Acids Res"; date: 
            "2016-12-15- 15"; volume: "44"; issue: "22"; pages: "10912-10928"
COMMENT     Sequence Label: pRS314_U6_U100C_U101C
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..49
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    166..187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(202..207,208..225,226..232)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    240..256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     245..267
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     264..280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     264..280
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        301..319
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     347..363
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     348..364
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(682..698)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(682..698)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
       61 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      121 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      181 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      241 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga
      301 aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga ggtcgacggt
      361 atcgataagc ttgatatcga attcttcggc tactataaat aaatgttttt ttcgcaacta
      421 tgtgttcgcg aagtaaccct tcgtggacat ttggtcaatt tgaaacaata cagagatgat
      481 cagcagttcc cctgcataag gatgaaccgt tttacaaaga gacctatttc gttttttttt
      541 atcatcgagt gaagtatcgt gacttgtaca tttgaagata cccagcgtac agcagtgtat
      601 ctttatcttc ctgtatgata tagataacta acatctcgaa tagaaaattg tctcgcgttc
      661 gaacctaagc ctttgctatc ggatccacta gttctagagc ggcc
//
