LOCUS       sequence-155738-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 
            2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). 
            Also contains bicistronially expressed cytoplasmic red reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: 2PH-PLCd-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..53
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(976..1000)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gatcccatcg attcgaattc aaggatcaac aagtttgtac aaaaaagcag gctgccgcca
       61 ccatggactc gggccgggac ttcctgaccc tgcacggcct acaggatgat gaggatctac
      121 aggcgctgct gaagggcagc cagctcctga aggtgaagtc cagctcatgg aggagagagc
      181 gcttctacaa gttgcaggag gactgcaaga ccatctggca ggagtcccgc aaggtcatgc
      241 ggaccccgga gtcccagctg ttctccatcg aggacattca ggaggtgcga atggggcacc
      301 gcacggaggg tctggagaag ttcgcccgtg atgtgcccga ggaccgctgc ttctccattg
      361 tcttcaagga ccagcgcaat acactagacc tcatcgcccc atcgccagct gatgcccagc
      421 actgggtgct ggggctgcac aaaattatcc atcacagcgg atctatggac cagcgtcaga
      481 agctacagca ctggattcac tcctgcttgc gaaaagctga caaaaacaag gacaacaaga
      541 taggcggcag cggcggcagc ggcggcgata gcggtagaga ttttctgact ctgcatggac
      601 tgcaggacga cgaggacctg caggccctgc tgaagggcag ccagctgctg aaggtgaaga
      661 gcagcagctg gcgccgcgag cgcttctaca agctgcagga ggactgcaag accatctggc
      721 aggagagccg caaggtgatg cgcacccccg agagccagct gttcagcatc gaggacatcc
      781 aggaggtgcg catgggccac cgcaccgagg gcctggagaa gttcgcccgc gacgtgcccg
      841 aggaccgctg cttcagcatc gtgttcaagg accagcgcaa caccctggac ctgatcgccc
      901 ccagccccgc cgacgcccag cactgggtgc tgggcctgca caagatcatt caccattctg
      961 gcagcatgga tgggtaccca gctttcttgt acaaagtggt cgacggaaag tcttacccaa
     1021 ctgtgagtgc tgattaccag gacgccgttg agaaggcgaa
//
