LOCUS       sequence-155740-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 and Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pRSF-DUET Cas1 Cas2
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    454..478
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(478..497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(479..497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(611..628)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc
      121 tgccatatca aacaggtaaa aaagacacca accttaaacc atccaaatct accggggtac
      181 gcctgtttaa cccaaatgtc tggaactcaa atcccgtttc cgtattcgtt gcccatgcca
      241 tcactacatt gccttcttcc gccagtccag ctatttgttc ccagatcatt tcacgaattt
      301 ttgcggatac atcacctaca tatacccctg cacgtacctc caacaaccag atggctaatc
      361 tgcctcgtaa gcgcggaggt acattttcag tgaccacgac caacatactc attgtatatc
      421 tccttcttat acttaactaa tatactaaga tggggaattg ttatccgctc acaattcccc
      481 tatagtgagt cgtattaatt tcgattatgc ggccgtgtac aatacgatta ctttctgttc
      541 gacttaagca ttatgcggcc gcaagcttgt cgacctgcag gcgcgccgag ctcgaattcg
      601 gatcctggct gtggtgatga tggtgatggc tgctgcccat gtcagctact ccgatggcct
      661 gcatctccca gtgaaacagg aagcggaatg gcaacaggct gtgcatcttc aggtggggcc
      721 ggcggttgta tttctccagc ggcaagcacg tcctctataa gcggaatcaa tttggctaat
      781 gttttactac tgcgaa
//
