LOCUS       sequence-155741-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 
            2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). 
            Also contains bicistronially expressed cytoplasmic red reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: 2PH-PLCd-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..111)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(121..867)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    872..896
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttcatgtgc atgttctcct taatcagctc gctcacggta ccgcttccag gagcaggacc
       61 ggggttttct tccacgtctc ctgcttgctt taacagagag aagttcgtgg ctccggatcc
      121 ggcatcagca aacccaagct cggaaagctt ttggtgagcc tcagcgtaat cagcaaagaa
      181 ggcatcttcg tccgctgcat atttgtcaac gagagggcgg aatacagggt cagacaaaag
      241 agccttgtca gaaggtagct gaaggagacc ttccttctca ccactcaaca actccgtgaa
      301 gtatgagttg tcgaaaataa gaggattaga ggtccaggga ccctcaaatc cagaacgctc
      361 cttgtgtgca gctccaatag tgtgaccccc agatagagca acgatatctt ggtcagtaag
      421 ccccatagct ttgccaaaca catctctcaa atggtcagaa cccttagtgg gatcgggcaa
      481 gcgaccctct ggtggtggct caggcttgtc ctctcttcca gggtggaatg gaaccttagg
      541 tccacccgtg acctcaacgg caacaacgcc agccaactgg tagaaatcgg cgtagctcaa
      601 aatagggaac tccgccttga gtggctccaa aagcctaaca gcgatgtcaa gaccgttgtt
      661 agcgctgtga gccagttcgg cagggtgctt gatggttccg aagggtccac cggtcttcgt
      721 gcccttgtca aaggttccag cagagtggaa tgccaaacgg agcattagag gagcgcatct
      781 cttctcagcg atgaagcctc tgagcttctt cttcgccttc tcaacggcgt cctggtaatc
      841 agcactcaca gttgggtaag actttccgtc gaccactttg tacaagaaag ctgggtaccc
      901 atccatgctg ccagaatggt gaatgatctt gtgcaggccc agcacccagt gctgggcgtc
      961 ggcggggctg ggggcgatca ggtccagggt gttgcgctgg tccttgaaca cgatgctgaa
     1021 gcagcggtcc tcgggca
//
