LOCUS       sequence-155744-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUR Cas2
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             342..386
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(424..442)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      438..485
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatgag tatgttggtc gtggtcactg
       61 aaaatgtacc tccgcgctta cgaggcagat tagccatctg gttgttggag gtacgtgcag
      121 gggtatatgt aggtgatgta tccgcaaaaa ttcgtgaaat gatctgggaa caaatagctg
      181 gactggcgga agaaggcaat gtagtgatgg catgggcaac gaatacggaa acgggatttg
      241 agttccagac atttgggtta aacaggcgta ccccggtaga tttggatggt ttaaggttgg
      301 tgtctttttt acctgtttga gcggccgcac tcgagtctgg taaagaaacc gctgctgcga
      361 aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc taggctgctg
      421 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      481 ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata
      541 aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga
      601 ccgggtcatc gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt
      661 tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg
      721 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct t
//
