LOCUS       sequence-155745-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUR Cas2
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    410..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(434..453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(435..453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        499..576
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(596..615)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agtgcggccg ctcaaacagg taaaaaagac accaacctta aaccatccaa
      121 atctaccggg gtacgcctgt ttaacccaaa tgtctggaac tcaaatcccg tttccgtatt
      181 cgttgcccat gccatcacta cattgccttc ttccgccagt ccagctattt gttcccagat
      241 catttcacga atttttgcgg atacatcacc tacatatacc cctgcacgta cctccaacaa
      301 ccagatggct aatctgcctc gtaagcgcgg aggtacattt tcagtgacca cgaccaacat
      361 actcatggta tatctcctta ttaaagttaa acaaaattat ttctacaggg gaattgttat
      421 ccgctcacaa ttcccctata gtgagtcgta ttaatttcct aatgcaggag tcgcataagg
      481 gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga
      541 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
      601 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
      661 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
      721 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
      781 tc
//
