LOCUS       sequence-155746-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for sgRNA and for Expression of Cas9 linked via 
            T2A to mCherry linked to the Ad4 E4orf6 gene via P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..104
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 agggccgcca ctccaccggc ggcatggacg agctgtacaa gctcgaggca acaaacttct
       61 cactactcaa acaagcaggt gacgtggagg agaatcccgg gcctcttaag atgtcaggaa
      121 atagtagcat catgactcgc tcacgcacaa gactggcact gtcaaggcac cacccatatc
      181 agccccccgc aactctgcct agatgcgagg aaacagagtc tcgggctagt ctggtggaag
      241 accaccctgt cctgccagac tgcgatactc tgagtatgca taacgtgagc tccgtccgcg
      301 gactgccctg ttcagcaggg ttcactgtgc tgcaggagtt tcccgtgcct tgggacatgg
      361 tcctgacccc tgaggaactg agggtgctga agacatgcat gtctgtctgt ctgtgctgtg
      421 ccaacattga tctgttctct agtcagctga tccacggaag agagaggtgg gtcatccact
      481 gccattgtca gaatcccggg agtctgagat gcatggccgg cggaacagtg ctggctatct
      541 ggttcaggag aatcattcag ggctgtatgt ttaaccagcg agtgatgtgg tacagggagg
      601 tggtcaatct gcatatgcca aaagaaatca tgtatatggg cagcgtgttc tggcgcggac
      661 gacacctgat ctacattcgg atctggtatg acgggcatgt gggctccatt gtcccccaga
      721 tgtcctttgg gtggtctact ctgaattacg gcctgctgaa caatctggtg g
//
