LOCUS       sequence-155749-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 and Cas2 E9Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 Cas2 E9Q
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgagtg
       61 cggccgctca aacaggtaaa aaagacacca accttaaacc atccaaatct accggggtac
      121 gcctgtttaa cccaaatgtc tggaactcaa atcccgtttc cgtattcgtt gcccatgcca
      181 tcactacatt gccttcttcc gccagtccag ctatttgttc ccagatcatt tcacgaattt
      241 ttgcggatac atcacctaca tatacccctg cacgtacctc caacaaccag atggctaatc
      301 tgcctcgtaa gcgcggaggt acattttgag tgaccacgac caacatactc atttcagcta
      361 ctccgatggc ctgcatctcc cagtgaaaca ggaagcggaa tggcaacagg ctgtgcatct
      421 tcaggtgggg ccggcggttg tatttctcca gcggcaagca cgtcctctat aagcggaatc
      481 aatttggcta atgttttact actgcgaaaa atatccctgc acgccaaacg gacttcccgg
      541 tccggctcac cagggttacg acgcgctatc tcaaaagctt tcggtacaac agtgtcaaat
      601 ttaatgatgt ctgcaatatc gtaaacaaag gaaagaggct ttcctgtatg cacaaaccca
      661 atagctggtg cataaccagc tgcaagtatc gccgcttcag ttacgccgta taaacaggaa
      721 gttgcagcgc taatgcattg gttgatcgta tcgccctttt cccagtcttt cggatcgtag
      781 cgacgtccat tccatg
//
