LOCUS       sequence-155753-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) 
            linked to IRES-mCherry under the control of TetOp and a minimal CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   McDonald JI, Celik H, Rois LE, Fishberger G, Fowler T, Rees R, 
            Kramer A, Martens A, Edwards JR, Challen GA
  TITLE     Reprogrammable CRISPR/Cas9-based system for inducing site-specific 
            DNA methylation.
  JOURNAL   Biol Open. 2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067.
  PUBMED    27170255
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2016 May 11. pii: bio.019067. doi: 10.1242/bio.019067."
COMMENT     Sequence Label: TetO-dCas9-D3A
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             118..138
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aagcggtaca cctctacgaa ggaggtcctg gacgccacac tgattcatca gtcaattacg
       61 gggctctatg aaacaagaat cgacctctct cagctcggtg gagacagcag ggctgacccc
      121 aagaagaaga ggaaggtggg agctagcgac tacaaagacg atgacgacaa gggaagccga
      181 gaggactggc cctcccggct ccagatgttc ttcgctaata accacgacca ggaatttgac
      241 cctccaaagg tttacccacc tgtcccagct gagaagagga agcccatccg ggtgctgtct
      301 ctctttgatg gaatcgctac agggctcctg gtgctgaagg acttgggcat tcaggtggac
      361 cgctacattg cctcggaggt gtgtgaggac tccatcacgg tgggcatggt gcggcaccag
      421 gggaagatca tgtacgtcgg ggacgtccgc agcgtcacac agaagcatat ccaggagtgg
      481 ggcccattcg atctggtgat tgggggcagt ccctgcaatg acctctccat cgtcaaccct
      541 gctcgcaagg gcctctacga gggcactggc cggctcttct ttgagttcta ccgcctcctg
      601 catgatgcgc ggcccaagga gggagatgat cgccccttct tctggctctt tgagaatgtg
      661 gtggccatgg gcgttagtga caagagggac atctcgcgat ttctcgagtc caaccctgtg
      721 atgattgatg ccaaagaagt gtcagctgca cacagggccc gctacttctg gggtaacctt
      781 cccggtatga acaggccgtt ggcatccact gtgaatgata agctggagct gcaggagtgt
      841 ctggagcatg gcaggatagc caagttcagc aaagtgagga ccattactac gaggtcaaac
      901 tccataaagc agggcaaaga ccagcatttt cctgtcttca tgaatgagaa agaggacatc
      961 ttatggtgca ctgaaatgga aagggtattt ggtttcccag tccactatac tgacgtctcc
     1021 aacatgagcc gcttggcgag gcagagactg ctgggccggt catgga
//
