LOCUS       sequence-155757-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FtsZ without a peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  TITLE     FtsZ-MEEVF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FtsZ
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..19
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3..19
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(35..52)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(35..51)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(44..66)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     209..227
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(251..265,266..271,272..288,289..295)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    271..287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             338..349
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg
       61 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
      121 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cctaagaaac
      181 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
      241 cctcgagaaa tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc
      301 aattgtgagc ggataacaat ttcacacaga attcattaaa gaggagaaat taactactag
      361 tatgtttgaa ccaatggaac ttaccaatga cgcggtgatt aaagtcatcg gcgtcggcgg
      421 cggcggcggt aatgctgttg aacacatggt gcgcgagcgc attgaaggtg ttgaattctt
      481 cgcggtaaat accgatgcac aagcgctgcg taaaacagcg gttggacaga cgattcaaat
      541 cggtagcggt atcaccaaag gactgggcgc tggcgctaat ccagaagttg gccgcaatgc
      601 ggctgatgag gatcgcgatg cattgcgtgc ggcgctggaa ggtgcagaca tggtctttat
      661 tgctgcgggt atgggtggtg gtaccggtac aggtgcagca ccagtcgtcg ctgaagtggc
      721 aaaagatttg ggtatcctga ccgttgctgt cgtcactaa
//
