LOCUS       sequence-155758-        1365 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FtsZ without a peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1365)
  TITLE     FtsZ-MEEVF
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FtsZ
FEATURES             Location/Qualifiers
     source          1..1365
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..52
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(76..90,91..96,97..113,114..120)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    128..144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             163..174
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
       61 cttcacctcg agaaatcata aaaaatttat ttgctttgtg agcggataac aattataata
      121 gattcaattg tgagcggata acaatttcac acagaattca ttaaagagga gaaattaact
      181 actagtatgt ttgaaccaat ggaacttacc aatgacgcgg tgattaaagt catcggcgtc
      241 ggcggcggcg gcggtaatgc tgttgaacac atggtgcgcg agcgcattga aggtgttgaa
      301 ttcttcgcgg taaataccga tgcacaagcg ctgcgtaaaa cagcggttgg acagacgatt
      361 caaatcggta gcggtatcac caaaggactg ggcgctggcg ctaatccaga agttggccgc
      421 aatgcggctg atgaggatcg cgatgcattg cgtgcggcgc tggaaggtgc agacatggtc
      481 tttattgctg cgggtatggg tggtggtacc ggtacaggtg cagcaccagt cgtcgctgaa
      541 gtggcaaaag atttgggtat cctgaccgtt gctgtcgtca ctaagccttt caactttgaa
      601 ggcaagaagc gtatggcatt cgcggagcag gggatcactg aactgtccaa gcatgtggac
      661 tctctgatca ctatcccgaa cgacaaactg ctgaaagttc tgggccgcgg tatctccctg
      721 ctggatgcgt ttggcgcagc gaacgatgta ctgaaaggcg ctgtgcaagg tatcgctgaa
      781 ctgattactc gtccgggttt gatgaacgtg gactttgcag acgtacgcac cgtaatgtct
      841 gagatgggct acgcaatgat gggttctggc gtggcgagcg gtgaagaccg tgcggaagaa
      901 gctgctgaaa tggctatctc ttctccgctg ctggaagata tcgacctgtc tggcgcgcgc
      961 ggcgtgctgg ttaacatcac ggcgggcttc gatctgcgtc tggatgagtt cgaaacggta
     1021 ggtaacacca tccgtgcatt tgcttccgac aacgcgactg tggttatcgg tacttctctt
     1081 gacccggata tgaatgacga gctgcgcgta accgttgttg cgacaggtat cggcatggac
     1141 aaacgtcctg aaatcactct ggtgaccaat aagcaggttc agcagccagt gatggatcgc
     1201 taccagcagc atgggatggc tccgctgacc caggagcaga agccggttgc taaagtcgtg
     1261 aatgacaatg cgccgcaaac tgcgaaagag ccggattatc tggatatccc agcattcctg
     1321 cgtaagcaag ctgatggcgg cagcggcagc ggcagcagct gataa
//
