LOCUS       sequence-155759-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses shRNA targeting TCF1 and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  TITLE     Rothenberg lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Banshee shTCF1 mCherry
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        322..570
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     322..342
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     493..512
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcggta gtcgacggat
      121 cctctagacg cgtctcgagt cgacggtatc gattagtcca atttgttaaa gacaggatat
      181 cagtggtcca ggctctagtt ttgactcaac aatatcacag ctgaagccta tagagtacga
      241 gccatagata gaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagatct
      301 ggatccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      361 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      421 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      481 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      541 ttatatatct tgtggaaagg acgaaacacc ggccacgagt ctgaacaata atttcaagag
      601 aattattgtt tagacttgtg gcttttttaa gcttggatca aggtcagaaa cagagagaca
      661 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
      721 agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc
      781 cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta
      841 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
      901 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc cc
//
