LOCUS       sequence-155762-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 V33A and Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 V33A Cas2
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agtgcggccg ctcaaacagg taaaaaagac accaacctta aaccatccaa
      121 atctaccggg gtacgcctgt ttaacccaaa tgtctggaac tcaaatcccg tttccgtatt
      181 cgttgcccat gccatcacta cattgccttc ttccgccagt ccagctattt gttcccagat
      241 catttcacga atttttgcgg atacatcacc tacatatacc cctgcacgta cctccaacaa
      301 ccagatggct aatctgcctc gtaagcgcgg aggtacattt tcagtgacca cgaccaacat
      361 actcatttca gctactccga tggcctgcat ctcccagtga aacaggaagc ggaatggcaa
      421 caggctgtgc atcttcaggt ggggccggcg gttgtatttc tccagcggca agcacgtcct
      481 ctataagcgg aatcaatttg gctaatgttt tactactgcg aaaaatatcc ctgcacgcca
      541 aacggacttc ccggtccggc tcaccagggt tacgacgcgc tatctcaaaa gctttcggta
      601 caacagtgtc aaatttaatg atgtctgcaa tatcgtaaac aaaggaaaga ggctttcctg
      661 tatgcacaaa cccaatagct ggtgcataac cagctgcaag tatcgccgct tcagttacgc
      721 cgtataaaca ggaagttgca gcgctaatgc attggttgat cgtatcgcc
//
