LOCUS       sequence-155764-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses inactive WspR G249A under a T7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pCOLA-T7-WspR:G249A
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        108..126
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    127..151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             207..224
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        338..356
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    357..381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
       61 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa tacgactcac
      121 tataggggaa ttgtgagcgg ataacaattc ccctgtagaa ataattttgt ttaactttaa
      181 taaggagata taccatgggc agcagccatc accatcatca ccacagccag gatccgaatt
      241 cgagctcggc gcgcctgcag gtcgacaagc ttgcggccgc ataatgctta agtcgaacag
      301 aaagtaatcg tattgtacac ggccgcataa tcgaaattaa tacgactcac tataggggaa
      361 ttgtgagcgg ataacaattc cccatcttag tatattagtt aagtataaga aggagatata
      421 catatgcaca accctcatga gagcaagacc gacctgggcg cccctctgga cggcgcggtc
      481 atggtactgc ttgtcgatga tcaggccatg atcggagagg ctgtgcgtcg ctcactggcg
      541 agcgaggcgg gcatcgactt ccatttctgt tccgatccgc agcaggcggt ggcggtggcc
      601 aaccagatca agccgacggt gatcctccag gacctggtga tgcccggcgt cgacggcctc
      661 acgctgctcg ctgcctaccg cggcaacccg gcgacccgcg acatcccgat catcgtcctg
      721 tcgaccaagg aagagccgac ggtgaagagc gcggcgttcg ccgccggcgc caacgactac
      781 ctggtcaagc tgccggacgc catcgagctg gtggcgcgga tccgctacca c
//
