LOCUS       sequence-155765-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression plasmid for kinase dead Plk4 with multi-phospho
            degron deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt
       61 aaacttaagc ttaccggtgc cgccaccatg gcgacctgca tcggggagaa gatcgaggat
      121 tttaaagttg gaaatctgct tggtaaagga tcatttgctg gtgtctacag agctgagtcc
      181 attcacactg gtttggaagt tgcaatcaaa atgatagata agaaagccat gtacaaagca
      241 ggaatggtac agagagtcca aaatgaggtg aaaatacatt gccaattgaa acatccttct
      301 atcttggagc tttataacta ttttgaagat agcaattatg tgtatctggt attagaaatg
      361 tgccataatg gagaaatgaa caggtatcta aagaatagag tgaaaccctt ctcagaaaat
      421 gaagctcgac acttcatgca ccagatcatc acagggatgt tgtatcttca ttctcatggt
      481 atactacacc gggacctcac actttctaac ctcctactga ctcgtaatat gaacatcaag
      541 attgctgctt ttgggctggc aactcaactg aaaatgccac atgaaaagca ctatacatta
      601 tgtggaactc ctaactacat ttcaccagaa attgccactc gaagtgcaca tggccttgaa
      661 tctgatgttt ggtccctggg ctgtatgttt tatacattac ttatcgggag accacccttc
      721 gacactgaca cagtcaagaa cacattaaat aaagtagtat tggcagatta tgaaatgcca
      781 tcttttttgt caatagaggc caaggacctt attcaccagt tacttcgtag aaatccagca
      841 gatcgtttaa gtctgtcttc agtattggac catcctttta tgtcccgaaa ttcttcaaca
      901 aaaagtaaag atttaggaac tgtgga
//
