LOCUS       sequence-155766-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression plasmid for Plk4 with multi-phospho degron 
            deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO Plk4 ∆24-mCherry
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
       61 taccggtgcc gccaccatgg cgacctgcat cggggagaag atcgaggatt ttaaagttgg
      121 aaatctgctt ggtaaaggat catttgctgg tgtctacaga gctgagtcca ttcacactgg
      181 tttggaagtt gcaatcaaaa tgatagataa gaaagccatg tacaaagcag gaatggtaca
      241 gagagtccaa aatgaggtga aaatacattg ccaattgaaa catccttcta tcttggagct
      301 ttataactat tttgaagata gcaattatgt gtatctggta ttagaaatgt gccataatgg
      361 agaaatgaac aggtatctaa agaatagagt gaaacccttc tcagaaaatg aagctcgaca
      421 cttcatgcac cagatcatca cagggatgtt gtatcttcat tctcatggta tactacaccg
      481 ggacctcaca ctttctaacc tcctactgac tcgtaatatg aacatcaaga ttgctgattt
      541 tgggctggca actcaactga aaatgccaca tgaaaagcac tatacattat gtggaactcc
      601 taactacatt tcaccagaaa ttgccactcg aagtgcacat ggccttgaat ctgatgtttg
      661 gtccctgggc tgtatgtttt atacattact tatcgggaga ccacccttcg acactgacac
      721 agtcaagaac acattaaata aagtagtatt ggcagattat gaaatgccat cttttttgtc
      781 aatagaggcc aaggacctta ttcaccagtt acttcgtaga aatccagcag atcgtttaag
      841 tctgtcttca gtattggacc atccttttat gtcccgaaat tcttcaacaa aaa
//
