LOCUS       sequence-155772-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression of Ct biosensor and WspR WT under individual T7 
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Wang XC, Wilson SC, Hammond MC
  TITLE     Next-generation RNA-based fluorescent biosensors enable anaerobic 
            detection of cyclic di-GMP.
  JOURNAL   Nucleic Acids Res. 2016 Jul 5. pii: gkw580.
  PUBMED    27382070
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Jul 5. pii: gkw580."
COMMENT     Sequence Label: pETDuet-T7-Ct-WspR
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      complement(72..118)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(361..380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(362..380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg acaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctatg
      121 gcgcccgaac agggacttga accctggacc cgcggccgga tgtaactagt tacggagctc
      181 acactctact caacaaatgg cagcctggcg atcatagctt taaaacattg ttttaaaaca
      241 gtagacccat ggctttgcgt catcaccttt cggtgatttt gccctgtttc attggacccg
      301 tccttcacca tttcattcag ttacatccgg ccgctgctct accgactgag ctatccgggc
      361 ccctatagtg agtcgtatta atttcgcggg atcgatttcg attatgcggc cgtgtacaat
      421 acgactttct gttcgactta agcatcagcc cgccggggcc ggcggcaccg gctgttccat
      481 caggcccacc tggttgcggc cattgttctt ggcctggtag agcgcctggt cggccatctc
      541 gatgagaacc cggaaggttt gcccgccgcc gcccggcacc agggtgctga cgccgatgct
      601 gacggtcagg tgcgagcccg ggcgtggctg gtcgtggctg atctgcaggc tctccaccgt
      661 gcgccggacc ttctccgcca gcaaccgtgc gccgcccggc gaggtgcccg ggaggaccat
      721 ggcgaactcc tcgccgccgt accgagccgc caggtccgag gagcgactgc agccctcgcg
      781 gatggccccg gcgacctggc gcagggcctc gtcgccggcg acgtggccga aggtgtcgtt
      841 gtagctcttg aaatagtcca cgtcgatcat cagcagcgac agttgcgact gctcgcgcag
      901 cgagcggcgc cactccatct ccaggtattc gtcgaagtga cgacggttgg agagcccggt
      961 caggccgtcg gagttcatca gccgctgcag caccaggttg gtctccagca actg
//
