LOCUS       sequence-155775-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual selection plasmid with lac operator, selection for binding with
            spectinomycin, for non-binding with chloramphenicol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Hoffmann SA, Kruse SM, Arndt KM
  TITLE     Long-range transcriptional interference in E. coli used to construct
            a dual positive selection system for genetic switches.
  JOURNAL   Nucleic Acids Res. 2016 Jun 2;44(10):e95. doi: 10.1093/nar/gkw125. 
            Epub 2016 Feb 29.
  PUBMED    26932362
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-06-2"; volume: "44(10):e95. doi"; pages: " 
            10.1093/nar/gkw125. Epub 2016 Feb 29"
COMMENT     Sequence Label: pSAH0016
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(118..909)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVIGK"
ORIGIN
        1 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
       61 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aaagctttta
      121 tttacctatt accttggtga tctcgccttt cacgtagtgg acaaattctt ccaactgatc
      181 tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag cttcaagtat
      241 gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga catccttcgg
      301 cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca ctacatttcg
      361 ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat ttagcgcctc
      421 aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac ctaccaaggc
      481 aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga tcgtggctgg
      541 ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca gttcgcgctt
      601 agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt ctacagcgcg
      661 gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga tcaaagctcg
      721 ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat cactgtgtgg
      781 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
      841 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
      901 ttccctcatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct
      961 gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccga
//
