LOCUS       sequence-155784-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 P202Q and Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 P202Q Cas2
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..61)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 tagctgcgct agtagacgag tccatgtgct ggcgttcaaa tttcgcagca gcggtttctt
       61 taccagactc gagtgcggcc gctcaaacag gtaaaaaaga caccaacctt aaaccatcca
      121 aatctaccgg ggtacgcctg tttaacccaa atgtctggaa ctcaaatccc gtttccgtat
      181 tcgttgccca tgccatcact acattgcctt cttccgccag tccagctatt tgttcccaga
      241 tcatttcacg aatttttgcg gatacatcac ctacatatac ccctgcacgt acctccaaca
      301 accagatggc taatctgcct cgtaagcgcg gaggtacatt ttcagtgacc acgaccaaca
      361 tactcatttc agctactccg atggcctgca tctcccagtg aaacaggaag cggaatggca
      421 acaggctgtg catcttcagg tggggccggc ggttgtattt ctccagcggc aagcacgtcc
      481 tctataagcg gaatcaattt ggctaatgtt ttactactgc gaaaaatatc cctgcacgcc
      541 aaacggactt cccggtccgg ctcaccaggg ttacgacgcg ctatctcaaa agctttcggt
      601 acaacagtgt caaatttaat gatgtctgca atatcgtaaa caaaggaaag aggctttcct
      661 gtatgcacaa acccaatagc ttgtgcataa ccagctgcaa gtatcgccgc ttcagttacg
      721 ccgtataaac aggaagttgc agcgctaa
//
