LOCUS       sequence-155785-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Grinkova YV, Denisov IG, Sligar SG
  TITLE     Engineering extended membrane scaffold proteins for self-assembly of
            soluble nanoscale lipid bilayers.
  JOURNAL   Protein Eng Des Sel. 2010 Nov . 23(11):843-8.
  PUBMED    20817758
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein Eng
            Des Sel. 2010 Nov . 23(11):843-8."
COMMENT     Sequence Label: pMSP2N2
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgggtcat catcatcatc atcatcacga
       61 ttatgatatt cctactactg agaatttgta ttttcagggt tctaccttca gtaaacttcg
      121 cgaacaactg ggccccgtga cgcaggaatt ctgggacaac ctggaaaaag aaaccgaggg
      181 actgcgtcag gaaatgtcca aagatttaga agaggtgaag gccaaggttc agccatatct
      241 cgatgacttt cagaaaaaat ggcaggaaga gatggaatta tatcgtcaaa aggtggaacc
      301 gctgcgtgcg gaactgcaag agggggcacg ccaaaaactc catgagctcc aagagaagct
      361 cagcccatta ggcgaagaaa tgcgcgatcg cgcccgtgca catgttgatg cactccggac
      421 tcatttggcg ccgtattcgg atgaacttcg ccagcgtttg gccgcacgtc tcgaggcgct
      481 gaaagaaaac gggggtgccc gcttggctga gtaccacgcg aaagcgacag aacacctgag
      541 caccttgagc gaaaaagcga aaccggcgct ggaagatcta cgccagggct tattgcctgt
      601 tcttgagagc tttaaagtca gttttctgtc agctctggaa gaatatacta aaaagctgaa
      661 tacccagggt acccccgtga cgcaggaatt ctgggacaac ctggaaaaag aaaccgaggg
      721 actgcgtcag gaaatgtcca aagatttaga agaggtgaag gccaaggttc agccatatct
      781 cgatgacttt cagaaaaaat ggcaggaaga gatggaatta tatcgtcaaa aggtggaacc
      841 gctgcgtgcg gaactgcaag aggg
//
