LOCUS       sequence-155787-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 P212L and Cas2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 P212L Cas2
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..59)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gctgcgctag tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta
       61 ccagactcga gtgcggccgc tcaaacaggt aaaaaagaca ccaaccttaa accatccaaa
      121 tctaccgggg tacgcctgtt taacccaaat gtctggaact caaatcccgt ttccgtattc
      181 gttgcccatg ccatcactac attgccttct tccgccagtc cagctatttg ttcccagatc
      241 atttcacgaa tttttgcgga tacatcacct acatataccc ctgcacgtac ctccaacaac
      301 cagatggcta atctgcctcg taagcgcgga ggtacatttt cagtgaccac gaccaacata
      361 ctcatttcag ctactccgat ggcctgcatc tcccagtgaa acaggaagcg gaatggcaac
      421 aggctgtgca tcttcaggtg gggccggcgg ttgtatttct ccagcggcaa gcacgtcctc
      481 tataagcgga atcaatttgg ctaatgtttt actactgcga aaaatatccc tgcacgccaa
      541 acggacttcc cggtccggct caccagggtt acgacgcgct atctcaaaag ctttcggtac
      601 aacagtgtca aatttaatga tgtctgcaat atcgtaaaca aaggaaagaa gctttcctgt
      661 atgcacaaac ccaatagctg gtgcataacc agctgcaagt atcgccgctt cagttacgcc
      721 gtataaacag gaagttgcag cgctaatgca ttgg
//
