LOCUS       sequence-155791-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas1 and Cas2 T72I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUKI Cas1 Cas2 T72I
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..56)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gcgctagtag acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca
       61 gactcgagtg cggccgctca aacaggtaaa aaagacacca accttaaacc atccaaatct
      121 accggggtac gcctgtttaa cccaaaaatc tggaactcaa atcccgtttc cgtattcgtt
      181 gcccatgcca tcactacatt gccttcttcc gccagtccag ctatttgttc ccagatcatt
      241 tcacgaattt ttgcggatac atcacctaca tatacccctg cacgtacctc caacaaccag
      301 atggctaatc tgcctcgtaa gcgcggaggt acattttcag tgaccacgac caacatactc
      361 atttcagcta ctccgatggc ctgcatctcc cagtgaaaca ggaagcggaa tggcaacagg
      421 ctgtgcatct tcaggtgggg ccggcggttg tatttctcca gcggcaagca cgtcctctat
      481 aagcggaatc aatttggcta atgttttact actgcgaaaa atatccctgc acgccaaacg
      541 gacttcccgg tccggctcac cagggttacg acgcgctatc tcaaaagctt tcggtacaac
      601 agtgtcaaat ttaatgatgt ctgcaatatc gtaaacaaag gaaagaggct ttcctgtatg
      661 cacaaaccca atagctggtg cataaccagc tgcaagtatc gccgcttcag ttacgccgta
      721 taaacaggaa gttgcagcgc taatgcattg gttgatcgta tcgcc
//
