LOCUS       sequence-155794-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding N-terminal fusions of 
            TagRFPt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: pME-TagRFPt-NS
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(834..933)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctgccacc atggtgagca agggagagga
      121 gctgatcaag gagaacatgc acatgaagct gtacatggag ggaacagtga acaaccacca
      181 cttcaagtgt acaagcgagg gagagggaaa gccttacgag ggaacacaga caatgagaat
      241 caaggtggtg gagggaggac ctctgccttt cgctttcgac atcctggcta caagcttcat
      301 gtacggaagc agaacattca tcaaccacac acagggaatc cctgacttct tcaagcagag
      361 cttccctgag ggattcacat gggagagagt gacaacatac gaggacggag gagtgctgac
      421 agctacacag gacacaagcc tgcaggacgg atgtctgatc tacaacgtga agatcagagg
      481 agtgaacttc cctagcaacg gacctgtgat gcagaagaag acactgggat gggaggctaa
      541 cacagagatg ctgtaccctg ctgacggagg actggaggga agaacagaca tggctctgaa
      601 gctggtggga ggaggacacc tgatctgtaa cttcaagaca acatacagaa gcaagaagcc
      661 tgctaagaac ctgaagatgc ctggagtgta ctacgtggac cacagactgg agagaatcaa
      721 ggaggctgac aaggagacat acgtggagca gcacgaggtg gctgtggcta gatactgtga
      781 cctgcctagc aagctgggac acaagctgaa cggaatggac gagctgtaca agcacccagc
      841 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
      901 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctat
//
