LOCUS       sequence-155796-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Shipman SL, Nivala J, Macklis JD, Church GM
  TITLE     Molecular recordings by directed CRISPR spacer acquisition.
  JOURNAL   Science. 2016 Jun 9. pii: aaf1175.
  PUBMED    27284167
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 9. pii: aaf1175."
COMMENT     Sequence Label: pWUR 1+2 tetO mut89
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..17
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 tttgtattac ctccttattt caagttaaac aaaattattt gtagagggaa accgttgtgg
       61 tctccctgaa tatagcctac gagccttatg catgcccgta aagttatcca gcaaccactc
      121 atagacctag ggcagcagat agggacgacg tggtgttagc tgtggtaaac gccatgggca
      181 ttaaaaaatg ccctcttggg ttatcaagag ggtcattata tttgctgagg agtgccgtta
      241 attaaggctc aaacaggtaa aaaagacacc aaccttaaac catccaaatc taccggggta
      301 cgcctgttta acccaaatgt ctggaactca aatcccgttt ccgtattcgt tgcccatgcc
      361 atcactacat tgccttcttc cgccagtcca gctatttgtt cccagatcat ttcacgaatt
      421 tttgcggata catcacctac atatacccct gcacgtacct ccaacaacca gatggctaat
      481 ctgcctcgta agcgcggagg tacattttca gtgaccacga ccaacatact catttcagct
      541 actccgatga cctgcatctc cctgtgaaac aggaagcgga atggcaacag gctgtgcatc
      601 ttcaggtggg gccggcggtt gtatatctcc agcggcaagc acgtcctcta taagcggaat
      661 caatttggct aatgttttac tactgcgaaa aatatccctg cacgccaaac ggacttcccg
      721 gtccggctca tcagggttac gacgcgctat ctcaaaagct ttcggtacaa cagtgtcaaa
      781 t
//
