LOCUS       sequence-155799-        4973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 
            scaffold domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pWW2149
FEATURES             Location/Qualifiers
     source          1..4973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1063..1092
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1108..1125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1181..1198)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1351..1397
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1539..2198
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1605..1624)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2219..2313
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2737..2754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2871..3416)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2951..2970)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccattagcgg cacgggatta gggctggcaa ttgtgcagcg tatcgtggat aaccataacg
       61 ggatgctgga gcttggcacc agcgagcggg gcgggctttc cattcgcgcc tggctgccag
      121 tgccggtaac gcgggcgcag ggcacgacaa aagaaggggg atctggtagt ggcagcggca
      181 gcggtagtgg atctggtagt ggcagcggca gcggtagtgg atctatggca gagtatgtgc
      241 gggccctgtt tgactttaat gggaatgatg aagaagacct tccctttaag aaaggagaca
      301 tcctgagaat ccgggataag cctgaagagc agtggtggaa tgcagaggac agcgaaggaa
      361 agagggggat gattcctgtc ccttacgtgg agaagtatgg atctggtagt ggcagcggca
      421 gcggtagtgg atctatggca gagtatgtgc gggccctgtt tgactttaat gggaatgatg
      481 aagaagacct tccctttaag aaaggagaca tcctgagaat ccgggataag cctgaagagc
      541 agtggtggaa tgcagaggac agcgaaggaa agagggggat gattcctgtc ccttacgtgg
      601 agaagtatgg atctggtagt ggcagcggca gcggtagtgg atctatggca gagtatgtgc
      661 gggccctgtt tgactttaat gggaatgatg aagaagacct tccctttaag aaaggagaca
      721 tcctgagaat ccgggataag cctgaagagc agtggtggaa tgcagaggac agcgaaggaa
      781 agagggggat gattcctgtc ccttacgtgg agaagtatgg atctggtagt ggcagcggca
      841 gcggtagtgg atctatggca gagtatgtgc gggccctgtt tgactttaat gggaatgatg
      901 aagaagacct tccctttaag aaaggagaca tcctgagaat ccgggataag cctgaagagc
      961 agtggtggaa tgcagaggac agcgaaggaa agagggggat gattcctgtc ccttacgtgg
     1021 agaagtatgg atcctgactc gagtaatgag aagcttgggc ccgaacaaaa actcatctca
     1081 gaagaggatc tgaatagcgc cgtcgaccat catcatcatc atcattgagt ttaaacggtc
     1141 tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
     1201 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     1261 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     1321 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     1381 tgggcctttc gttttatctg ttgtttgtcg gtgaactaat ttctagactg cagttgatcg
     1441 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     1501 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1561 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1621 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1681 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1741 atgctcatcc ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1801 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1861 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1921 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1981 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     2041 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     2101 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     2161 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tgatatcgag ctcgcttgga
     2221 ctcctgttga tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc
     2281 ggttgccgcc gggcgttttt tattggtgag aatccaagcc tcggtgagaa tccaagcctc
     2341 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     2401 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     2461 cgtcgggtga tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca
     2521 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
     2581 ggcaaaagca ccgccggaca tcagcggtag cggagtgtat actggcttac tatgttggca
     2641 ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg
     2701 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc
     2761 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     2821 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     2881 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     2941 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     3001 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     3061 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     3121 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     3181 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     3241 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     3301 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     3361 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     3421 atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg
     3481 tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgtttg acagcttatc
     3541 atcgataagc ttccgatggc gcgccgagag gctttacact ttatgcttcc ggctgaattc
     3601 actagtttga cagttagctc agtcctagga attatgctag cggtacctcc cggtgccctc
     3661 taccagggcc aagagatcta tgattaaccg tatccgcgta gtcacgctgt tggtaatggt
     3721 gctgggggta ttcgcactgt tacagcttat ttccggcagt ctgttttttt cttcccttca
     3781 ccatagccag aagagctttg tggtttccaa tcaattacgg gaacagcagg gcgagctgac
     3841 gtcaacctgg gatttaatgc tgcaaacgcg cattaacctg agtcgttcag cggtacggat
     3901 gatgatggat tcctccaatc aacaaagtaa cgccaaagtt gaattgctcg atagcgccag
     3961 gaaaacattg gcgcaggcag cgacgcatta taaaaaattc aaaagcatgg caccgttacc
     4021 tgaaatggtc gctaccagtc gtaatattga tgaaaaatat aaaaactatt acacagcgtt
     4081 aactgaactg attgattatc tagattatgg caatactgga gcttatttcg ctcagccaac
     4141 ccagggaatg caaaatgcaa tgggcgaagc gtttgctcag tacgccctca gcagtgaaaa
     4201 actgtatcgc gatatcgtca ctgacaacgc agatgattac cgatttgccc agtggcaact
     4261 ggcggttatc gcgctggtgg tggtattgat tctgctggtg gcgtggtacg gcattcgccg
     4321 tatgttgctt actccgctgg caaaaattat tgctcacatt cgcgaaatcg ccggtggtaa
     4381 cctggcgaat accctgacca ttgacgggcg cagtgaaatg ggcgacctgg cgcagagcgt
     4441 ttcacatatg gcggctggtg ttaagcaact ggcggatgac cgcacgctgc tgatggcggg
     4501 ggtaagtcac gacttgcgca cgccgctgac gcgtattcgc cttgcgactg agatgatgag
     4561 cgagcaggat ggctatctgg cagaatcgat caataaagat atcgaagagt gcaacgccat
     4621 cattgagcag tttatcgact acctgcgcac cgggcaggag atgccgatgg aaatggcgga
     4681 tcttaatgca gtactcggtg aggtgattgc tgccgaaagt ggctatgagc gggaaattga
     4741 aaccgcgctt taccccggca gcattgaagt gaaaatgcac ccgctgtcga tcaaacgcgc
     4801 ggtggcgaat atggtggtca acgccgcccg ttatggcaat ggctggatca aagtcagcag
     4861 cggaacggag ccgaatcgcg cctggttcca ggtggaagat gacggtccgg gaattgcgcc
     4921 ggaacaacgt aagcacctgt tccagccgtt tgtccgcggc gacagtgcgc gca
//
