LOCUS       sequence-155815-         279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains bicistronic pTet-Scaffold(3xFLAG)- ShineDelgarno-mCherry 
            and TetR genes. Used for Western blots..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH011
FEATURES             Location/Qualifiers
     source          1..279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             214..279
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cctccacctg cattaccgcc aaagagaagg cgtggatctg ggtccggcag cggtggatct
       61 atcacaattc gggcagcttt tctggagaag gaaaacaccg cactgcgtac tgagattgct
      121 gaactggaga aagaagtagg ccgctgcgag aatattgtta gcaaatatga aacccgctac
      181 ggccccctgg gatccggtag tggcagcggc agcgactaca aggaccatga cggtgattac
      241 aaggatcatg acatcgacta caaggatgac gatgacaag
//
