LOCUS       sequence-155816-        3672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTet-Scaffold with no fluorescent protein. Used as a control for 
            autofluorescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH002
FEATURES             Location/Qualifiers
     source          1..3672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(138..764)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     669..688
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        780..835
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    816..834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             993..1010
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1266..1295
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1311..1328
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1384..1401)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1554..1600
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1642..1659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1813..2401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1893..1912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2572..3363,3364..3432))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3195..3214
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3433..3537)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(3627..3670)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcacta gtgatccgtt tccatttagg tgggtacgtt ggagccgcat tattttcgct
       61 ttatgaatct aaagggtggt taactcgaca tcttggttac cgtgaagtta ccatcacgga
      121 aaaaggttat gctgctttta agacccactt tcacatttaa gttgtttttc taatccgcat
      181 atgatcaatt caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg
      241 atagcttgtc gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta
      301 gcgacttgat gctcttgatc ttccaatacg caacctaaag taaaatgccc cacagcgctg
      361 agtgcatata atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt
      421 tcgagagttt catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg
      481 cgatgactta gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag
      541 ctttcccctt ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag
      601 gcgtcgagca aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct
      661 agcttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct
      721 aatgcgctgt taatcacttt acttttatct aatcttgaca tcattaattc ctaatttttg
      781 ttgacactct atcgttgata gagttatttt accactccct atcagtgata gagaaaagtg
      841 aaaatccata tgactagcag atccactaga gtcgactaag aaaccattat tatcatgaca
      901 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacgctag cggtacctga
      961 agtgtggccc aacgcaaact aagagatcta tgcatcacca tcaccatcac gactacgaca
     1021 tcccgactac cgaaaacctg tacttccagc ctccacctgc attaccgcca aagagaaggc
     1081 gtggatctgg gtccggcagc ggtggatcta tcacaattcg ggcagctttt ctggagaagg
     1141 aaaacaccgc actgcgtact gagattgctg aactggagaa agaagtaggc cgctgcgaga
     1201 atattgttag caaatatgaa acccgctacg gccccctgaa ctcgagtaat gagaagcttg
     1261 ggcccgaaca aaaactcatc tcagaagagg atctgaatag cgccgtcgac catcatcatc
     1321 atcatcattg agtttaaacg gtctccagct tggctgtttt ggcggatgag agaagatttt
     1381 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
     1441 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
     1501 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
     1561 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaact
     1621 aatttctaga ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1681 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1741 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1801 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1861 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1921 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1981 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2041 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2101 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2161 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2221 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2281 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2341 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2401 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2461 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2521 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2581 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2641 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2701 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2761 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2821 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2881 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2941 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3001 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3061 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3121 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3181 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3241 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3301 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3361 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3421 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3481 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3541 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3601 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     3661 ggatgatttc tg
//
