LOCUS       sequence-155824-       11810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base vector targeting genes to the GAPDH locus of human cells. 
            Inserted genes are expressed from a 2A sequence at the C terminus of
            GAPDH. Puromycin resistance is encoded by the Muro gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris
            FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E,
            Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ,
            Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG
  TITLE     GAPTrap: A Simple Expression System for Pluripotent Stem Cells and 
            Their Derivatives.
  JOURNAL   Stem Cell Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015.
  PUBMED    27594589
REFERENCE   2  (bases 1 to 11810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2016 Sep 1. pii: S2213-6711(16)30139-4. doi: 
            10.1016/j.stemcr.2016.07.015."
COMMENT     Sequence Label: pGAPTrap-IRESMuro
FEATURES             Location/Qualifiers
     source          1..11810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             5581..5634
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(5657..6231,6232..6234,6235..6243)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5824..5841)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6051..6070
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(11166..11185)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11167..11185)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11195..11212)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11195..11211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11204..11226)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(11352..11807)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11489..11510)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11701..11720
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggtacgttaa ttaaaatggg
     2221 gaaggtgaag gtcggagtca acgggtgagt tcgcgggtgg ctggggggcc ctgggctgcg
     2281 accgcccccg aaccgcgtct acgagccttg cgggctccgg gtctttgcag tcgtatgggg
     2341 gcagggtagc tgttccccgc aaggagagct caaggtcagc gctcggacct ggcggagccc
     2401 cgcacccagg ctgtggcgcc ctgtgcagct ccgcccttgc ggcgccatct gcccggagcc
     2461 tccttcccct agtccccaga aacaggaggt ccctactccc gcccgagatc ccgacccgga
     2521 cccctaggtg ggggacgctt tctttccttt cgcgctctgc ggggtcacgt gtcgcagagg
     2581 agcccctccc ccacggcctc cggcaccgca ggccccggga tgctagtgcg cagcgggtgc
     2641 atccctgtcc ggatgctgcg cctgcggtag agcggccgcc atgttgcaac cgggaaggaa
     2701 atgaatgggc agccgttagg aaagcctgcc ggtgactaac cctgcgctcc tgcctcgatg
     2761 ggtggagtcg cgtgtggcgg ggaagtcagg tggagcgagg ctagctggcc cgatttctcc
     2821 tccgggtgat gcttttccta gattattctc tggtaaatca aagaagtggg tttatggagg
     2881 tcctcttgtg tcccctcccc gcagaggtgt ggtggctgtg gcatggtgcc aagccgggag
     2941 aagctgagtc atgggtagtt ggaaaaggac atttccaccg caaaatggcc cctctggtgg
     3001 tggccccttc ctgcagcgcc ggctcacctc acggccccgc ccttcccctg ccagcctagc
     3061 gttgacccga ccccaaaggc caggctgtaa atgtcaccgg gaggattggg tgtctgggcg
     3121 cctcggggaa cctgcccttc tccccattcc gtcttccgga aaccagatct cccaccgcac
     3181 cctggtctga ggttaaatat agctgctgac ctttctgtag ctgggggcct gggctggggc
     3241 tctctcccat cccttctccc cacacacatg cacttacctg tgctcccact cctgatttct
     3301 ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa gccctgggac tagggggtta
     3361 aaatacagct tcccctcttc ccacccgccc cagtctctgt cccttttgta ggagggactt
     3421 agagaagggg tgggcttgcc ctgtccagtt aatttctgac ctttactcct gccctttgag
     3481 tttgatgatg ctgagtgtac aagcgttttc tccctaaagg gtgcagctga gctaggcagc
     3541 agcaagcatt cctggggtgg catagtgggg tggtgaatac catgtacaaa gcttgtgccc
     3601 agactgtggg tggcagtgcc ccacatggcc gcttctcctg gaagggcttc gtatgactgg
     3661 gggtgttggg cagccctgga gccttcagtt gcagccatgc cttaagccag gccagcctgg
     3721 cagggaagct caagggagat aaaattcaac ctcttgggcc ctcctggggg taaggagatg
     3781 ctgcattcgc cctcttaatg gggaggtggc ctagggctgc tcacatattc tggaggagcc
     3841 tcccctcctc atgccttctt gcctcttgtc tcttagattt ggtcgtattg ggcgcctggt
     3901 caccagggct gcttttaact ctggtaaagt ggatattgtt gccatcaatg accccttcat
     3961 tgacctcaac tacatggtga gtgctacatg gtgagcccca aagctggtgt gggaggagcc
     4021 acctggctga tgggcagccc cttcataccc tcacgtattc ccccaggttt acatgttcca
     4081 atatgattcc acccatggca aattccatgg caccgtcaag gctgagaacg ggaagcttgt
     4141 catcaatgga aatcccatca ccatcttcca ggagtgagtg gaagacagaa tggaagaaat
     4201 gtgctttggg gaggcaacta ggatggtgtg gctcccttgg gtatatggta accttgtgtc
     4261 cctcaatatg gtcctgtccc catctccccc ccacccccat aggcgagatc cctccaaaat
     4321 caagtggggc gatgctggcg ctgagtacgt cgtggagtcc actggcgtct tcaccaccat
     4381 ggagaaggct ggggtgagtg caggagggcc cgcgggaggg gaagctgact cagccctgca
     4441 aaggcaggac ccgggttcat aactgtctgc ttctctgctg taggctcatt tgcagggggg
     4501 agccaaaagg gtcatcatct ctgccccctc tgctgatgcc cccatgttcg tcatgggtgt
     4561 gaaccatgag aagtatgaca acagcctcaa gatcatcagg tgaggaaggc agggcccgtg
     4621 gagaagcggc cagcctggca ccctatggac acgctcccct gacttgcgcc ccgctccctc
     4681 tttctttgca gcaatgcctc ctgcaccacc aactgcttag cacccctggc caaggtcatc
     4741 catgacaact ttggtatcgt ggaaggactc atggtatgag agctggggaa tgggactgag
     4801 gctcccacct ttctcatcca agactggctc ctccctgccg gggctgcgtg caaccctggg
     4861 gttgggggtt ctggggactg gctttcccat aatttccttt caaggtgggg agggaggtag
     4921 aggggtgatg tggggagtac gctgcagggc ctcactcctt ttgcagacca cagtccatgc
     4981 catcactgcc acccagaaga ctgtggatgg cccctccggg aaactgtggc gtgatggccg
     5041 cggggctctc cagaacatca tccctgcctc tactggcgct gccaaggctg tgggcaaggt
     5101 catccctgag ctgaacggga agctcactgg catggccttc cgtgtcccca ctgccaacgt
     5161 gtcagtggtg gacctgacct gccgtctaga aaaacctgcc aaatatgatg acatcaagaa
     5221 ggtggtgaag caggcgtcgg agggccccct caagggcatc ctgggctaca ctgagcacca
     5281 ggtggtctcc tctgacttca acagcgacac ccactcctcc acctttgacg ctggggctgg
     5341 cattgccctc aacgaccact ttgtcaagct catttcctgg tatgtggctg gggccagaga
     5401 ctggctctta aaaagtgcag ggtctggcgc cctctggtgg ctggctcaga aaaagggccc
     5461 tgacaactct tttcatcttc taggtatgac aacgaatttg gctacagcaa cagggtggtg
     5521 gacctcatgg cccacatggc ctccaaggag ggaatggcgc gtggcggcgg gtccggagga
     5581 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccggcgcgc
     5641 caagtcgacg gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     5701 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5761 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5821 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5881 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5941 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     6001 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     6061 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     6121 atctggggcc tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     6181 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     6241 accatggcgc gtatggcaag actgaccgaa tacaagccta ccgtgagact ggcaacaaga
     6301 gatgatgtcc caagagccgt gcgaaccctg gcagcagcat tcgcagacta cccagccaca
     6361 agacacactg tggaccccga taggcatatc gagagagtga ccgagctgca ggaactgttt
     6421 ctgacacggg tgggcctgga tattggaaag gtctgggtgg cagatgatgg ggctgctgtg
     6481 gccgtctgga caaccccaga gtctgtggaa gctggagctg tcttcgctga gatcggccct
     6541 cgaatggcag aactgtctgg ctccaggctg gcagcacagc agcagatgga gggactgctg
     6601 gcaccacaca gacctaagga accagcttgg ttcctggcaa ctgtgggggt ctctcccgac
     6661 catcagggca aaggactggg gagtgccgtg gtcctgcctg gagtggaggc tgcagaaaga
     6721 gctggagtcc cagcttttct ggagacatct gccccccgga acctgccttt ctatgaacgc
     6781 ctgggcttta ctgtgaccgc tgacgtggag gtgcccgaag gcccccgaac ttggtgcatg
     6841 acccgaaagc caggagcata aatctagagg cgcgctatcg ataagcttag atcgagcaca
     6901 agaggaagag agagaccctc actgctgggg agtccctgcc acactcagtc ccccaccaca
     6961 ctgaatctcc cctcctcaca gttgccatgt agaccccttg aagaggggag gggcctaggg
     7021 agccgcacct tgtcatgtac catcaataaa gtaccctgtg ctcaaccagt tacttgtcct
     7081 gtcttattct agggtctggg gcagagggga gggaagctgg gcttgtgtca aggtgagaca
     7141 ttcttgctgg ggagggacct ggtatgttct cctcagactg agggtagggc ctccaaacag
     7201 ccttgcttgc ttcgagaacc atttgcttcc cgctcagacg tcttgagtgc tacaggaagc
     7261 tggcaccact acttcagaga acaaggcctt ttcctctcct cgctccagtc ctaggctatc
     7321 tgctgttggc caaacatgga agaagctatt ctgtgggcag ccccagggag gctgacaggt
     7381 ggaggaagtc agggctcgca ctgggctctg acgctgactg gttagtggag ctcagcctgg
     7441 agctgagctg cagcgggcaa ttccagcttg gcctccgcag ctgtgaggtc ttgagcacgt
     7501 gctctattgc tttctgtgcc ctcgtgtctt atctgaggac atcgtggcca gcccctaagg
     7561 tcttcaagca ggattcatct aggtaaacca agtacctaaa accatgccca aggcggtaag
     7621 gactatataa tgtttaaaaa tcggtaaaaa tgcccacctc gcatagtttt gaggaagatg
     7681 aactgagatg tgtcagggtg acttatttcc atcatcgtcc ttaggggaac ttgggtaggg
     7741 gcaaggcgtg tagctgggac ctaggtccag acccctggct ctgccactga acggctcagt
     7801 tgctttgggc agttactccc gggcctcact ttgcacgtgt gcttacctag tggagacaaa
     7861 agtacatacc tcggtagagc gcgcacgcct gtaaccccag cactttggga ggccaaggtg
     7921 ggtgtatcac ctgaggtcag gagtttgaga ccagcctggc caacatggtg aaactccgtc
     7981 tctactaaaa ttacaaaaat cagccaggct tcatggcaca tgcctatagt cccagctaca
     8041 ggcatgctga agcaggagaa tcgcttgcac cccggaggca gaggctgcag tgagctgaga
     8101 ccacaccact gcactccagc ctaggcaaca gagtatgaga ctccatctca aaaaaaaaaa
     8161 aagtacctac ctcagagttc aaactagtga atattaggaa gtgcttgaga cagtgacacc
     8221 aaagtgcaca ataaatactc gccagtttca ttattattaa agaatccatt tgaatgtcag
     8281 ctcaacacag cctcctatac cgaggcattg tgaaccgcat ctccccagct tctccaggct
     8341 tttccaagaa tcagggacac tgtagcctgt tggtctcagt gtatgacaga cacggaggaa
     8401 gcacatcttt agctgatact taaacagaga ccctgagcgc acatacaccc gcgcacacat
     8461 gcatggagct tcaccttctc tgtcattctg cagtgaccag gagagcaaga gctcccacct
     8521 cccttcaaaa cactgtgccc atcccgggca ctaaggcctc tttaaagcac ggcacctcca
     8581 cgagggaggg ccacagccac atacactcca cctggcaggt ggacagcgtg agcacgtgga
     8641 ccatagcagg gacaaggtgc cccggccagc cccaacgccc tctgccgctg acagggacag
     8701 aagccctctc cagctgcgtg tgctgcagag gccatgcgta gcctccagct gcattctatt
     8761 ccactccagt gcctgggcca gttagcacca gtgtggaaga cagtgagctg gctccggaca
     8821 acagggatgg aggaaaggtc ccacattcac attcctgata cgtggacaag gtgaggggcc
     8881 gcaatcgctc tggcagcatt ttaaagatgg ggaagtagca gacacccacg cgtgaaggca
     8941 ggagagcccc aactgtggtg gaaatggccc cagaatggta gggccaagcc tagctccaga
     9001 caccccagag ccctggagaa gccaagactg agggagaaag cctgagggag gagcgcccca
     9061 gtccccaggg accggcctgg tgcagagctg cagctgatgt tcccctctgt gcagccccac
     9121 cctctgcctc gctgagctcc ctgctgcgag ggcctcgggt gcaaggggga ggcaggtctc
     9181 tatctcatgg agctgtcaga tgagacatcg cgatcggagt cctcagcctc gcttggcggc
     9241 ggcggcgggt cgctaagcgg gaccgcagtg aaagcaggag actttctaga aaaaaacacc
     9301 agttgtcaac cttggggcag gcaggaatcc tgaagacgga cggcactcct cctcctgctg
     9361 cctcaccctc tggcagcccg tgagaagtac cggaagcgag ggcggggccg cgggatggcg
     9421 agggagcggc agggactgaa ctctctccaa acccaccctg acagggaaat gggccccgcc
     9481 tgtgtcttgg gaactcagag gctgaggtca ggcatgatgg ctcacgcctg taattccagc
     9541 actttgggag gcagaggcgg gtggatcacg acgtcaggag ttcaaggcaa gcctggccaa
     9601 catggtgaaa ccccatctct actaaaaatg caaaaattag ccaggtgtgg tggcgggcgc
     9661 cttggaggct gaggcaagat aatcacttga acctgggagg cggaggttgt agtgagccaa
     9721 aaaaaaaaaa aaaagaaata gctgaagtca cagtaggaga gaagctgctg agcctccagc
     9781 accctgactc tagggccttg gctttatgtc tatctgcagt atttttgtga tttttaaaaa
     9841 ttcactttct tgttgcggtg taacttacac agggtcaaat gcacaaatca tggccctgac
     9901 tttagataaa aatctgcccc cacaaccctt ctgttccttg ccagttttta aactgcctct
     9961 aaccagggga accaccagag ctggtggcct tgggaggttt cagccctccc gtcatgaatg
    10021 gacatagctc atccaactgc caagggagag agctgtgggt ctgggccagc cccaccaggt
    10081 aactcccaaa gggcagcccc acagcaagat gtgacccagt cattgcctga gggtctctgg
    10141 ggctgtgttc caacctttct ccccgctgtg tccccctgga aggccccatg cccaggggag
    10201 gcgcctaccg gtctccagac tgggtgatga gccggcggca ggtctccatc tgcacgtcca
    10261 ggccgcgctt catgctgcac atctccatgt actcgtgcag gtgccggttc atgtcgttct
    10321 tggccgtggc caactccagc tgtggtgggg caggcagggc catcggggtt aacaggtggc
    10381 gttcacagcg cctctgttgc ccccgccagg aggccaacac gccaagagca gtggctgggc
    10441 cgggggccca ggcagccatt actgaaggct cagattttaa aataaaccag accaaggcag
    10501 aaggcagcga atacaccatt gtttcctctg cttttcctgg aaatctttgc ctatggagga
    10561 atgcaaaggt actgctgcag gctgtgttct tttaagactg ataagagtgg tgaggaaagt
    10621 cagtgtgggc gggatggagc agatccacag gctcccaggg ccacacaggt tcctatgggg
    10681 cccggctcac ccctcatccc ctgtactgcg ctggtcctgg gctgtcccct cactgcactg
    10741 tgagctcccg taagggttag cgacaggttt tcctcatcct ggcagaccca gacctcgaag
    10801 ggaattaact ctctctgtga cccgtggcat acaagcctgg ctaactgctg aaaggggccc
    10861 aatcctctta ccattctctc cttatcactt tattttctca cctaaaagcc attttcagac
    10921 actaaaagga agactcccat attggaggtg ccattgattc tgagacctgt cctgatttca
    10981 gaaatgtcac acgtggaaaa cacggggctc agaatcaaag aaatccggtc tccctccccg
    11041 ggcccattct gcagcccaca ctctgaggca ggcggagagg cagggcccag gcagcacaaa
    11101 ctcagtgcca gggacagtcg attaattaag cgatcgcagg ccgcccgcgg tggagctcca
    11161 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
    11221 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
    11281 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
    11341 atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
    11401 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
    11461 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
    11521 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
    11581 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
    11641 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
    11701 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
    11761 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
//
