LOCUS       sequence-155836-        6727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent Reporter for Dopaminergic Neuron Differentiation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Oh MS, Hong SJ, Huh Y, Kim KS
  TITLE     Expression of transgenes in midbrain dopamine neurons using the 
            tyrosine hydroxylase promoter.
  JOURNAL   Gene Ther. 2009 Mar;16(3):437-40. doi: 10.1038/gt.2008.148. Epub 
            2008 Sep 18.
  PUBMED    18800154
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2008.148"; journalName: "Gene Ther"; date: "2009-03"; 
            volume: "16"; issue: "3"; pages: "437-40"
COMMENT     Sequence Label: pAAV2.5-THP-GFP
FEATURES             Location/Qualifiers
     source          1..6727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      2694..2703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2700..2702,2703..2705,2706..3419)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2700..2702,2703..2705,2706..3416)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2745..2766)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3006..3025)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3353..3374
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3474..3950
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3673..3692)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3990..4130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3990..4119
                     /label=AAV2 ITR
     rep_origin      4205..4660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4292..4311)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4502..4523
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4677..4696)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4796..4818
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4856..4874)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4942..5046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5047..5115,5116..5907)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5265..5284)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6078..6666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6567..6586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgaggcctc ttgggattct tctcatcaaa aagggcatgg
      181 gacacagcta aagcgtccag ggctcctctg tgcccacaga tgccttagat cttggcacaa
      241 tgtagtcagc cagctccgtg tgtgtgtgtg tgtgtttgca tgtatctcac agacagtgca
      301 caatggcctg gatgtgaaca gaggcaagag tctgggccag cagttgtctc ccaggagggt
      361 ccaaagacat cgtattttca agtttaggcc aggtgctcac ttgggtgagc tcagacacag
      421 acaaaggtct ggagagcaca cattccccac ccccacccag ctcctatgca agcacctcca
      481 gccgagacaa gaaaacgaat taaaaagcaa tatttgtgtc agcgtaagac atttgccgaa
      541 aggttaaatc cacactcgtg gtgctgcaca gcagccccct gtgcaggatt tgttaggcac
      601 agctccctcc taccccgtgc cacctgagca aatgccaggc tgggtgggct ggaaccagcc
      661 tgggcttgcc tcaccctgga atccccagca ccctccaaag gaggaccctg ggagtgggca
      721 tagacgccct tcaggtgtgg gcaacagccc ccagtcctca ggatgaaagg ctaaggtgca
      781 gccagctctg ccttcacggt gggaatgtct ctatgtgagc cctttctggg ctgtgaagaa
      841 cgctctgaga agggtcctgg gaccctggat aggccagagc tgtgctgggc atgtagagac
      901 aggagtgggc taaagcagca aaggcactga ccaaggaaga gttcagagag gagcgtggaa
      961 tatggggagg ggttcatagt aagagagagc aggcagtgga gagtaaatag tcactgagcc
     1021 ggggtttatg gggtttgtag gagcttactc agagaaagta gatgagagat gccatgccag
     1081 tctgagtatc acagagcccc aggctctcct gggaacggaa ctgtgagggc cagaaggtca
     1141 gcaagggagg ttagggagag ttccttttgt actgactcag catttatcct gctcccaggg
     1201 ggcaatgggg gccagtgagg gatgcagagc aaggcagtga tgtggcaggc agttcctgtt
     1261 gtgaaagagc tgggaaggga gcgggctggg cctggtacgt acagcaggcc atttctgagg
     1321 gtccgagtgc tgtctaggag gtgcagtgag acttcagtga tcagccagaa cagaagctaa
     1381 gcggggtggg gactgcgagt tcaggcttct gggtcttgca aatatccaga atgctaaatc
     1441 ctcagaaccc cagggtggcc attttcagag tgggttttgt cctttgggca cttgtgcaga
     1501 ctccaatatc cagagggata aggatggtac tcttcagtac ccttagtgag aggacacttt
     1561 tctctgaagg gcttgaatgt gccgagccat tacctgaagg aaggaaatga ctccagggac
     1621 ataggatggg cccagcacaa ctcacctgct acagagaaag gtcccctccc tggtctcctt
     1681 agagatcctg tttccctggc tgaggaagct agggtggatc tttgtgtaag tgggtgtgga
     1741 tgctaactgg aaaacaaaag gtcacttact gttagacctc ggggtaccat ggaagagatg
     1801 atcactgagt gtgcccttac atggggacca gctgagaatg gggctaccac tagctcgaga
     1861 ccatgataca gggaataagt gtgcatttgg gggtagggag tggctcagaa tactcttaac
     1921 caaagcagag gtttgctccc acaggaaggt gaggtcagaa ggccttaggg agctgccagg
     1981 ggctagggtt ggcaccatct cccaggctgt gtctttaagg agatgataat cagagggata
     2041 gaaccttgca aaagtgggcc agtcttggga atactataga ggaatagcct tctggaacat
     2101 tctgtgtctc ataggacctg cctggggatc cagccccagt gccagcacat ataccgactg
     2161 gggcagtgaa tagatagtac actttgttac atgggctggg gggaacatgg cccatgtcct
     2221 ggaggggact ttatgacaga catccaaaaa tccagtgaga gggcttctag atttgtctcc
     2281 aaaggttata gttctaacat gagcccttag gaaatccagc atggttctcc ctgtgtgccc
     2341 tggtttggtt agagagctct agcggtctcc tgtcccacag aataccagcc agcccctgcc
     2401 ctacgtcgtg cctcgggctg agggtgattc agaggcaggt gcctgtgaca gtggatgcaa
     2461 ttagatctaa tgggacggag gcctttctcg tcgccctcgc tccatgccca cccccgcctc
     2521 cctcaggcac agcaggcgtg gagaggatgc gcaggaggta ggaggtgggg gacccagagg
     2581 ggctttgacg tcagcctggc ctttaaagag ggcgcctgcc tggcgagggc tgtggagaca
     2641 gaactcggga ccaccagagg aattcgagct cggtacccgg ggatcctcta gacgccacca
     2701 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2761 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2821 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2881 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2941 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3001 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3061 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3121 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3181 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3241 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3301 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3361 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
     3421 ctagagtcga cctgcagaag cttgcctcga gcagcgctgc tcgagagatc tacgggtggc
     3481 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     3541 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     3601 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     3661 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3721 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     3781 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     3841 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     3901 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
     3961 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     4021 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4081 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4141 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4201 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4261 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4321 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4381 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4441 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4501 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     4561 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4621 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4681 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4741 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4801 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4861 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4921 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4981 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5041 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5101 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5161 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5221 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5281 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5341 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5401 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5461 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5521 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5581 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5641 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5701 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5761 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5821 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5881 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5941 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6001 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6061 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6121 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6181 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6241 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6301 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6361 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6421 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6481 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6541 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6601 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6661 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6721 cacatgt
//
