LOCUS       sequence-155840-         226 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3x NFAT binding sequence upstream of a luciferase reporter; can be 
            used to assay signal transduction through Ca2+, calcineurin and NFAT
            in any tissue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Clipstone NA, Crabtree GR
  TITLE     Identification of calcineurin as a key signalling enzyme in 
            T-lymphocyte activation.
  JOURNAL   Nature. 1992 Jun 25. 357(6380):695-7.
  PUBMED    1377362
REFERENCE   2  (bases 1 to 226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1992 Jun 25. 357(6380):695-7."
COMMENT     Sequence Label: pGL3-NFAT luciferase
FEATURES             Location/Qualifiers
     source          1..226
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(69..182)
                     /label=minimal IL-2 promoter
                     /label=IL-2 promoter
                     /note="minimal promoter from the human interleukin-2 gene"
     protein_bind    190..219
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
ORIGIN
        1 ggcctttctt tatgtttttg gcgtcttcca tggtggcttt accaacagta ccggaatgcc
       61 aagcttgggc aggagttgag gttactgtga gtagtgatta aagagagtga tagggaactc
      121 ttgaacaaga gatgcaattt atactgttaa ttctggaaaa atattatggg ggtgtcaaaa
      181 tgtcctcgag gaggaaaaac tgtttcatac agaaggcgtg gaggaa
//
