LOCUS       sequence-155845-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     Lentiviral vector for constitutive gene expression.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: N174-MCS (Puro)
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..655
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(56..75)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     552..572
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(725..745)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tsggckgttc tctcttctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac
       61 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
      121 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
      181 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
      241 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
      301 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
      361 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
      421 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
      481 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
      541 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
      601 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgggc
      661 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
      721 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
      781 ttgcttcccg tatgggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
      841 twwtgaggag ttgtggcccc gttgtcagca cmgkkgggcg tggtkgkkgc mmctgkkgtt
      901 ttgctgamsc aaccccccmm ytgtgt
//
