LOCUS       sequence-155852-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter11 with BCD1-gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Linshiz G, Stawski N, Goyal G, Bi C, Poust S, Sharma M, Mutalik V, 
            Keasling JD, Hillson NJ
  TITLE     PR-PR: cross-platform laboratory automation system.
  JOURNAL   ACS Synth Biol. 2014 Aug 15;3(8):515-24. doi: 10.1021/sb4001728. 
            Epub 2014 Jan 14.
  PUBMED    25126893
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001728";
            journalName: "ACS Synth Biol"; date: "2014-08-15- 15"; volume: "3"; 
            issue: "8"; pages: "515-24"
COMMENT     Sequence Label: pProm11_BCD1-GFP
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(3..710,711..713))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELY"
     CDS             complement(3..713)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELY"
     primer_bind     complement(40..60)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     655..683
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttgtagagct catccatgcc atgtgtaatc ccagcagcag ttacaaactc aagaaggacc
       61 atgtggtcac gcttttcgtt gggatctttc gaaaggacag attgtgtcga caggtaatgg
      121 ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct
      181 agttgaacgg aaccatcttc aacgttgtgg cgaattttga agttagcttt gattccattc
      241 ttttgtttgt ctgccgtgat gtatacattg tgtgagttaa agttgtactc gagtttgtgt
      301 ccaagaatgt ttccatcttc tttaaaatca atacccttta actcgatacg attaacaagg
      361 gtatcacctt caaacttgac ttcagcacgc gtcttgtagg tcccgtcatc tttgaaagat
      421 atagtgcgtt cctgtacata accttcgggc atggcactct tgaaaaagtc atgccgtttc
      481 atgtgatccg gataacggga aaagcattga acaccatagg tcagagtagt gacaagtgtt
      541 ggccacggaa caggtagttt tccagtagtg caaataaatt taagggtgag ttttccgttt
      601 gtagcatcac cttcaccctc tccacggaca gaaaatttgt gcccattaac atcaccatct
      661 aattcaacaa gaattgggac aactccagtg aaaagttctt ctcctttgct cattagaaag
      721 tctcctgtgc atgattaaga tgtttcagta cgaaaattgc tttcattgtt gatctccttt
      781 ttaagtgaac ttgggccctc cacaaacact aagagccgga tgattaattg tcaagttatg
      841 cagcaacgac tcatagaaag cacgcagctg atagggtcga cgtggtgttt gctgtgcgtt
      901 ggacgtgtgg attgggatga aaataccccc ataaaaaaag gcgccgaagc gccttaatac
      961 gttattagcc gatatacgta tactc
//
