LOCUS       sequence-155857-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  membrane-targeted biosensor for monitoring changes in intracellular 
            chloride.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Watts SD, Suchland KL, Amara SG, Ingram SL
  TITLE     A sensitive membrane-targeted biosensor for monitoring changes in 
            intracellular chloride in neuronal processes.
  JOURNAL   PLoS One. 2012;7(4):e35373. doi: 10.1371/journal.pone.0035373. Epub 
            2012 Apr 10.
  PUBMED    22506078
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(4):e35373. doi"; pages: " 
            10.1371/journal.pone.0035373. Epub 2012 Apr 10"
COMMENT     Sequence Label: mbYFPQS
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     CDS             join(165..167,168..170,171..683)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSQNVYIMADKQKNGIK
                     SNFKIRHNIE"
     primer_bind     complement(210..231)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(471..490)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     818..839
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagttct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgctg tgctgtatga
      121 gaagaaccaa acaggttgaa aagaatgatg aggaccaaaa gatcatggtg agcaagggcg
      181 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      241 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      301 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
      361 gctacggcct gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
      421 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      481 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      541 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      601 acaacagcca gaacgtctat atcatggccg acaagcagaa gaacggcatc aagagcaact
      661 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      721 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
      781 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      841 ccgccgccg
//
