LOCUS       sequence-155865-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to construct AAV-based targeting vectors that carry an 
            IVS-2Aneo cassette for promoter-trapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Karnan S, Ota A, Konishi Y, Wahiduzzaman M, Hosokawa Y, Konishi H
  TITLE     Improved methods of AAV-mediated gene targeting for human cell lines
            using ribosome-skipping 2A peptide.
  JOURNAL   Nucleic Acids Res. 2015 Dec 10. pii: gkv1338.
  PUBMED    26657635
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Dec 10. pii: gkv1338."
COMMENT     Sequence Label: pAAV-2Aneo
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          58..287
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     270..289
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             348..401
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(459..478)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 attatacgaa gttatggaat ttacccaatt cgctgtctgc gagggccagc tgttggggtg
       61 agtactccct ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg
      121 aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcgtccatct
      181 ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc
      241 catacacttg agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca
      301 ggtccaactg cattccggaa cgcgttgtac atccggacgc gtgtacagag ggcagaggaa
      361 gtcttctaac atgcggtgac gtggaggaga atcccggccc aaccatggct gaacaagatg
      421 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
      481 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
      541 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
      601 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
      661 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
      721 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
      781 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
      841 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
      901 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
      961 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttt
//
