LOCUS       sequence-155875-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cpstFRET actin sensor for mechanical force measurements in 
            cytoskeleton.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Guo J, Wang Y, Sachs F, Meng F
  TITLE     Actin stress in cell reprogramming.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):E5252-61. doi: 
            10.1073/pnas.1411683111. Epub 2014 Nov 24.
  PUBMED    25422450
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1411683111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-9- 9"; volume: "111"; issue: "49"; pages: "E5252-61"
COMMENT     Sequence Label: Actin-cpstFRET-Actin
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..38
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(102..104,105..617)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(144..165)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(405..424)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
       61 gcatggacga gctgtacaag gaattcggcg gcggtggcgg ggtgagcaag ggcgaggagc
      121 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      181 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
      241 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
      301 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      361 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      421 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      481 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      541 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
      601 tccgccacaa catcgaggac ggcggcagat ctatgggcag cgtgcagctc gccgaccact
      661 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gc
//
