LOCUS       sequence-155878-        6139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter plasmid for PE OCT4 enhancer activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Gafni O, Weinberger L, Mansour AA, Manor YS, Chomsky E, Ben-Yosef D,
            Kalma Y, Viukov S, Maza I, Zviran A, Rais Y, Shipony Z, Mukamel Z, 
            Krupalnik V, Zerbib M, Geula S, Caspi I, Schneir D, Shwartz T, Gilad
            S, Amann-Zalcenstein D, Benjamin S, Amit I, Tanay A, Massarwa R, 
            Novershtern N, Hanna JH
  TITLE     Derivation of novel human ground state naive pluripotent stem cells.
  JOURNAL   Nature. 2013 Dec 12;504(7479):282-6. doi: 10.1038/nature12745. Epub 
            2013 Oct 30.
  PUBMED    24172903
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature12745"; journalName: "Nature"; date: "2013-12-12- 
            12"; volume: "504"; issue: "7479"; pages: "282-6"
COMMENT     Sequence Label: pGL3-human OCT4 PE-SV40-Luc
FEATURES             Location/Qualifiers
     source          1..6139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    83..131
                     /note="synthetic polyadenylation signal"
     misc_feature    145..236
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     185..204
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1420..1616
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1467..1602
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1646..1655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1652..3304
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1718..1736)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3345..3466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3356..3375)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3410..3429
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3714..3731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3885..4473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3965..3984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4644..5435,5436..5504))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5267..5286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5505..5609)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5636..6091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5723..5742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5933..5954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gggcctcttc gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac
       61 gggaggtact tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
      121 ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa
      181 caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg
      241 ataggtaccg gatactcagg ccaggcccag aaaaacagac cctgaagggg agcttagggc
      301 agccttcctg cacccctcca caaatcactc gccacctcct ctgcgtcttt ctgccagcca
      361 gccccactaa acaaagcaca tccctcaatc ttccgggctc ggggagggat gcacgatgaa
      421 gctggacgcc tgagttcccc agaggaagga ggaactagat acctaggtcc ctgtaggggg
      481 cccttggtgc ccgtctgagg ctcagtcttt gaggggattg cagagggggg ttgctggagc
      541 tccttttagc gtctctgaag gggattctgt gtgaggggat tgggactggg gggttgggga
      601 gcaggaagca gtccccaggg gagccatcca ggcccattca agggttgagc acttgtttag
      661 ggttagagct gccccctctg gggaccggga ttgtccagcc aaggccattg tcctgccccc
      721 ttcccccagt ccctcccagg cttctttgaa cctgaagtca gatatttttt ctccacaccc
      781 cccaccccct ggttttcccc acccagggcc tagggctgga ggcctgggcc agggaggtgg
      841 gggagggaga acggggccta ccgtggtatt agatgtctga gttttggttg agaggggagc
      901 aaggaacctg atgtgcaggt tccatagtgg agggggccca aagcgggtgt cttatcactc
      961 tgtttcagca aaggttggga aactgaggcc caatcagtcc aaagtctggt cccttgaagg
     1021 ggaagtaggg gccaacccct tagtctgtta gatgaggaga gtctggagtc tgattctgga
     1081 agacggaggg gtggggggat ggggggtggg gggatatagc acggaggcct tgtctggcag
     1141 tctactcttg aagatggggt gaaatttggc aggctgggca gatggtgcca ggcacccagg
     1201 ctgcggggtg gctggatttg gccagtatcg ggatgggaat gcctaggatt ctggatggat
     1261 cgggggaagg cataagggag cagctggcca ttgtgcttat ggctgttgat gcattgaggg
     1321 atagcgccac acacacattc aataaatttg aggagctgag agggtgactg gcccctgaag
     1381 gcacagtgcc agaggtctgt ggactcgaga tctgcgatct gcatctcaat tagtcagcaa
     1441 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     1501 ctccgcccca tcgctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     1561 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     1621 ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca taaagaaagg
     1681 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     1741 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     1801 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     1861 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     1921 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     1981 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
     2041 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
     2101 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     2161 tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa
     2221 gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct
     2281 gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca
     2341 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     2401 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     2461 agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac
     2521 cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     2581 cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag
     2641 gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct
     2701 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     2761 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact
     2821 gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     2881 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     2941 acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc
     3001 tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc
     3061 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     3121 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     3181 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     3241 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt
     3301 gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
     3361 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3421 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     3481 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     3541 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
     3601 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     3661 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
     3721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     5641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5701 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5821 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5941 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6061 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
     6121 tgggaagggc gatcggtgc
//
