LOCUS       sequence-155881-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Liberti WA 3rd, Markowitz JE, Perkins LN, Liberti DC, Leman DP, 
            Guitchounts G, Velho T, Kotton DN, Lois C, Gardner TJ
  TITLE     Unstable neurons underlie a stable learned behavior.
  JOURNAL   Nat Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405.
  PUBMED    27723744
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405."
COMMENT     Sequence Label: pHAGE-RSV-GCAMP6f
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             29..61
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(65..73,74..88)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             116..172
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     385..406
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(512..533)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(773..792)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggttctcat catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg
       61 tcgggatctg tacgacgatg acgataagga tctcgccacc atggtcgact catcacgtcg
      121 taagtggaat aagacaggtc acgcagtcag agctataggt cggctgagct cactcgagaa
      181 cgtctatatc aaggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca
      241 caacatcgag gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg
      301 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca aactttcgaa
      361 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
      421 cactctcggc atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagcaaggg
      481 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      541 ccacaagttc agcgtgtccg gcgagggtga gggcgatgcc acctacggca agctgaccct
      601 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      661 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      721 caagtccgcc atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg
      781 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      841 gctgaa
//
