LOCUS       sequence-155883-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Liberti WA 3rd, Markowitz JE, Perkins LN, Liberti DC, Leman DP, 
            Guitchounts G, Velho T, Kotton DN, Lois C, Gardner TJ
  TITLE     Unstable neurons underlie a stable learned behavior.
  JOURNAL   Nat Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405.
  PUBMED    27723744
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Oct 10. doi: 10.1038/nn.4405."
COMMENT     Sequence Label: pHAGE-RSV-tdTomato-2A-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(174..230)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      242..251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(258..272,273..281))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(285..317)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(321..338)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(348..401)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(497..520)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(695..714)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgcacgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
       61 tgctggtagt ggtaggcgag ctgcacgccg ccgtcctcga tgttgtggcg gatgtggaag
      121 ttcgccttga tgccgttctt ctgcttgtcg gccttgatat agacgttctc gagtgagctc
      181 agccgaccta tagctctgac tgcgtgacct gtcttattcc acttacgacg tgatgagtcg
      241 accatggtgg cgagatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca
      301 ccagtcatgc tagccatacc atgatgatga tgatgatgag aacccatagg gccgggattc
      361 tcctccacgt caccgcatgt tagaagactt cctctgccct ctccgctgcc agacttgtac
      421 agctcgtcca tgccgtacag gaacaggtgg tggcggccct cggagcgctc gtactgttcc
      481 acgatggtgt agtcctcgtt gtgggaggtg atgtccagct tggtgtccac gtagtagtag
      541 ccgggcagtt gcacgggctt cttggccatg tagatggtct tgaactccac caggtagtgg
      601 ccgccgtcct tcagcttcag ggcctggtgg atctcgccct tcagcacgcc gtcgcggggg
      661 tacaggcgct cggtggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
      721 ggggggaagt tggtgccgcg catcttcacc ttgtagatca gcgtgccgtc ctgcagggag
      781 gagtcctggg tcacggtcac cagaccgccg tcctcgaagt tcatcacgcg ctcccacttg
      841 aag
//
